In vitro and in vivo toxicity assessment of alginate/eudragit S 100-enclosed chitosan-calcium phosphate-loaded iron saturated bovine lactoferrin nanocapsules (Fe-bLf NCs).
In vitro and in vivo toxicity assessment of alginate/eudragit S 100-enclosed chitosan-calcium phosphate-loaded iron saturated bovine lactoferrin nanocapsules (Fe-bLf NCs).
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DOI：
10.1016/j.biopha.2017.10.121
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Khoo Miew Leng;S. Vijayarathna;S. L. Jothy;S. Sasidharan;J. Kanwar
中科院分区：
文献类型：
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作者：
Khoo Miew Leng;S. Vijayarathna;S. L. Jothy;S. Sasidharan;J. Kanwar
众所周知，乳铁蛋白具有抗菌特性。本研究通过体外黄素实验研究了海藻酸盐/EUDRAGIT®s100封闭壳聚糖-磷酸钙负载Fe-bLf纳米胶囊（NCs）的毒性。对卤虾的致死试验表明，NCs的lc50值大于1 mg/mL，说明NCs对卤虾没有毒性。阳性对照重铬酸钾24 h lc50值为64.15 μg/mL，显示出对盐水对虾的毒性作用。MTT细胞毒性试验显示，NCs对非癌Vero细胞株无毒性，ic50值为536 μg/mL。彗星试验对Vero细胞的遗传毒性研究表明，无彗尾或短彗尾的nc在100 μg/mL时无显著的遗传毒性。在125、250、500和1000 μg/mL作用24 h的实验中，实验条件下NCs没有明显的遗传毒性作用。结果表明，去离子水处理的葱尖细胞与nc处理的葱尖细胞有丝分裂指数无显著差异（p >0.05）。总之，在本研究中未观察到NCs的毒性。因此，无毒的NCs作为一种治疗药物具有良好的发展潜力。
Lactoferrin has been known to have antimicrobial properties. This research was conducted to investigate the toxicity of Alginate/EUDRAGIT® S 100-enclosed chitosan–calcium phosphate-loaded Fe-bLf nanocapsules (NCs) byin vitroandin vivoassays. Brine shrimp lethality assay showed that the LC50value of NCs was more than 1 mg/mL which indicated that NCs was not toxic to Brine shrimp. However, the LC50values for the positive control potassium dichromate at 24 h is 64.15 μg/mL, which was demostrated the toxic effect against the brine shrimp. MTT cytotoxicity assay also revealed that NCs was not toxic against non-cancerous Vero cell line with IC50values of 536 μg/mL. Genotoxicity studies by comet assay on Vero cells revealed that NCs exerted no significant genotoxic at 100 μg/mL without tail or shorter comet tail.Allium ceparoot assay carried out at 125, 250, 500 and 1000 μg/mL for 24 h revealed that the NCs was destitute of significant genotoxic effect under experimental conditions. The results show that there is no significant difference (p >0.05) in mitotic index between the deionized water and NCs treatedAllium ceparoot tip cells. In conclusion, no toxicity was observed in NCs in this study. Therefore, nontoxic NCs has the good potential to develop as a therapeutic agent.