ULTRASTRUCTURE OF THE 1ST COMPONENT OF HUMAN-COMPLEMENT - ELECTRON-MICROSCOPY OF THE CROSSLINKED COMPLEX
ULTRASTRUCTURE OF THE 1ST COMPONENT OF HUMAN-COMPLEMENT - ELECTRON-MICROSCOPY OF THE CROSSLINKED COMPLEX
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DOI：
10.1073/pnas.79.2.586
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
SCHUMAKER, VN
中科院分区：
文献类型：
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作者：
STRANG, CJ;SIEGEL, RC;SCHUMAKER, VN
电子显微照片显示了人类补体的第一个成分 (C1)，它与水溶性碳二亚胺交联，以防止解离成 Clq 和 Clr2Cls2 亚基。在电子显微照片中可以看到交联分子的两个投影，称为顶部和轮廓。在这两个视图中，Clq 的头部都是可见的。从顶部看，Clr2Cls2 四聚体亚基似乎位于 Clq 的中心，并折叠形成紧凑的质量，遮盖了大部分臂和中心束。从侧面看，四聚体似乎位于 Clq 头和中央束之间的臂区域。在这些剖面投影中，头部和杆状中心束似乎都不含 Clr2Cls2。有时可以在 Clq 头部区域计数超过 6 个结构域，就好像四聚体的一部分已展开并在头部中突出一样。
Electron micrographs are shown of the 1st component of human complement (C1) which was crosslinked with a water-soluble carbodiimide to prevent dissociation into its Clq and Clr2Cls2 subunits. Two projections of the crosslinked molecule are seen in the electron micrographs, which are called top and profile. In both views, the Clq heads are visible. From the top, the Clr2Cls2 tetrameric subunit appears to be located centrally on the Clq and folded to form a compact mass obscuring most of the arms and central bundle. In profile, the tetramer appears to be located in the region of the arms between the Clq heads and the central bundle. Both the heads and the rod-like central bundle appear to be free of Clr2Cls2 in these profile projections. Sometimes it is possible to count more than 6 domains in the region of the Clq heads, as though a portion of the tetramer had unfolded to protrude among the heads.