Aggregation-Induced Electrochemiluminescence Immunosensor Based on 9,10-Diphenylanthracene Cubic Nanoparticles for Ultrasensitive Detection of Aflatoxin B1.
Aggregation-Induced Electrochemiluminescence Immunosensor Based on 9,10-Diphenylanthracene Cubic Nanoparticles for Ultrasensitive Detection of Aflatoxin B1.
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DOI：
10.1021/acsabm.0c01201
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发表时间：
2020-12
影响因子：
4.7
通讯作者：
X. Lv;Xiaoyun Xu;T. Miao;X. Zang;Chao Geng;Yanping Li;Bo Cui;Yishan Fang
中科院分区：
文献类型：
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作者：
X. Lv;Xiaoyun Xu;T. Miao;X. Zang;Chao Geng;Yanping Li;Bo Cui;Yishan Fang
采用再沉淀法合成了具有聚集诱导发光特性（AIEgens）的9，10-二苯基蒽立方纳米粒子（DPA CNPs），并将其修饰于玻碳电极上，在三正丙胺（TPA）存在下，观察到明亮稳定的电化学发光（ECL）信号。这种现象归因于具有受限运动的分子极大地阻止了激发态弛豫期间的能量泄漏，这促进了能量以光子形式的发射。此外，在聚集状态下的DPA CNPs的尺寸限制效应有效地增强了ECL发射。具有AIE特性的DPA CNPs在电化学发光免疫传感器中的应用尚未见报道。在这方面的贡献，自由标记的聚集诱导的电化学发光免疫传感器的基础上的DPA CNPs的制作和一个简单的战略，提出了超灵敏检测黄曲霉毒素B1（AFB 1）。黄曲霉毒素B1在0.01 ~ 100 ng/mL范围内呈线性猝灭，检出限为3 fg/mL。该传感器灵敏度高、准确度好、抗干扰能力强、稳定性好，可用于核桃样品中黄曲霉毒素B1的检测。因此，所制备的免疫传感器在食品、医药等领域具有重要的应用价值。
9,10-Diphenylanthracene cubic nanoparticles (DPA CNPs) with aggregation-induced emission characteristic (AIEgens) were synthesized through a facile reprecipitation method; then, a bright and stable electrochemiluminescence (ECL) signal can be observed when the DPA CNPs were modified at the glassy carbon electrode (GCE) in the presence of Tri-n-propylamine (TPA). This phenomenon is ascribed to the molecules with restricted movement that greatly blocked the energy leakage during the relaxation of the excited state, which facilitated the emission of energy in the form of photons. In addition, the size confinement effect of DPA CNPs in the aggregated state effectively enhanced the ECL emission. The application of DPA CNPs with AIE characteristics in an electrochemiluminescence immunosensor has not been reported. In this contribution, a free-label aggregation-induced electrochemiluminescence immunosensor based on DPA CNPs was fabricated and a simple strategy for ultrasensitive detection of aflatoxin B1 (AFB1) was proposed. The ECL signal is quenched linearly in the range of 0.01 pg/mL to 100 ng/mL for AFB1, and the detection limit is 3 fg/mL. In summary, the prepared sensor exhibits high sensitivity, acceptable accuracy, good anti-interference ability and stability, and satisfactory detection toward AFB1 in walnut samples. Therefore, the fabricated immunosensor will have significant applications in the fields of food, medicine, and so on.