The MADS-Box factor CeMEF2 is not essential for Caenorhabditis elegans myogenesis and development
The MADS-Box factor CeMEF2 is not essential for Caenorhabditis elegans myogenesis and development
复制标题
DOI：
10.1006/dbio.2000.9758
复制
发表时间：
2000-07-15
影响因子：
2.7
通讯作者：
Krause, M
中科院分区：
文献类型：
--
作者：
Dichoso, D;Brodigan, T;Krause, M
MEF 2是一种进化上保守的MADS（MCM 1，Agamous，Deficiens，and serum response factor）盒型转录因子，在脊椎动物和果蝇的肌肉发生中起关键作用。我们已经解决了单一的MEF 2样因子，CeMEF 2，在秀丽隐杆线虫的发展作用。使用表达测定和两个mef-2缺失等位基因，我们表明CeMEF 2是不需要适当的肌发生或发展。此外，一个假定的无效mef-2等位基因不能增强或抑制CeMyoD或CeTwist中突变体的表型。我们的研究结果表明，尽管其进化保守的序列和DNA结合特性，CeMEF 2已通过了不同的作用，在发展中的线虫相比，果蝇和脊椎动物。
MEF2 is an evolutionarily conserved MADS (MCM1, Agamous, Deficiens, and serum response factor) box-type transcription factor that plays a critical role in vertebrate and Drosophila melanogaster myogenesis. We have addressed the developmental role of the single MEF2-like factor, CeMEF2, in Caenorhabditis elegans. Using expression assays and two mef-2 deletion alleles, we show that CeMEF2 is not required for proper myogenesis or development. Moreover, a putative null mef-2 allele fails to enhance or suppress the phenotypes of mutants in CeMyoD or CeTwist. Our results suggest that despite its evolutionary conservation of sequence and DNA binding properties, CeMEF2 has adopted a divergent role in development in the nematode compared with Drosophila and vertebrates.