Comparative Transcriptomics Reveals Distinct Gene Expressions of a Model Ciliated Protozoan Feeding on Bacteria-Free Medium, Digestible, and Digestion-Resistant Bacteria
Comparative Transcriptomics Reveals Distinct Gene Expressions of a Model Ciliated Protozoan Feeding on Bacteria-Free Medium, Digestible, and Digestion-Resistant Bacteria
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比较转录组学揭示了以无细菌培养基、可消化和抗消化细菌为食的纤毛原生动物模型的独特基因表达
DOI：
10.3390/microorganisms8040559
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发表时间：
2020-04
期刊：
影响因子：
4.5
通讯作者：
Gong Jun
中科院分区：
文献类型：
--
作者：
Zou Songbao;Zhang Qianqian;Gong Jun
食菌作用是原生生物在自然生态系统中的重要生态功能。然而，有不同的细菌物种抵抗原生生物的消化，这减少了碳流到更高的营养水平。到目前为止，异养原生生物在捕食不同消化率的细菌猎物过程中的代谢过程的分子生物学观点仍然缺乏。本文研究了四膜虫在无菌超级蛋白胨（SPP）培养基（对照组）、消化抗性菌（DRB）和易消化大肠杆菌（E. coli（ECO）。我们发现原生生物种群在SPP中增长最快，在DRB处理中最慢。荧光原位杂交证实，确实有未消化，活菌在纤毛虫细胞与DRB喂养，但没有在其他治疗。RNA-seq数据的比较分析表明，相对于对照，T.在DRB和ECO处理中，嗜热菌分别显著和差异表达。在两种细菌处理中，溶酶体蛋白酶（特别是木瓜蛋白酶样半胱氨酸蛋白酶），GH 18几丁质酶和异柠檬酸裂解酶的protistan表达上调。与ECO相比，DRB处理中编码蛋白酶、糖苷酶和涉及糖酵解、TCA和乙醛酸循环的碳代谢过程的基因表达量更高。然而，谷胱甘肽代谢的基因在对照中比在两种细菌处理中的基因更多地上调，而不管细菌的消化率如何。本研究的结果表明，不仅细菌的食物，而且细菌类群的消化调节异养原生生物的多个代谢过程，这有助于更好地了解原生生物的噬菌性和细菌-原生生物的相互作用的分子基础上。
Bacterivory is an important ecological function of protists in natural ecosystems. However, there are diverse bacterial species resistant to protistan digestion, which reduces the carbon flow to higher trophic levels. So far, a molecular biological view of metabolic processes in heterotrophic protists during predation of bacterial preys of different digestibility is still lacking. In this study, we investigated the growth performance a ciliated protozoan Tetrahymena thermophila cultivated in a bacteria-free Super Proteose Peptone (SPP) medium (control), and in the media mixed with either a digestion-resistant bacterial species (DRB) or a digestible strain of E. coli (ECO). We found the protist population grew fastest in the SPP and slowest in the DRB treatment. Fluorescence in situ hybridization confirmed that there were indeed non-digested, viable bacteria in the ciliate cells fed with DRB, but none in other treatments. Comparative analysis of RNA-seq data showed that, relative to the control, 637 and 511 genes in T. thermophila were significantly and differentially expressed in the DRB and ECO treatments, respectively. The protistan expression of lysosomal proteases (especially papain-like cysteine proteinases), GH18 chitinases, and an isocitrate lyase were upregulated in both bacterial treatments. The genes encoding protease, glycosidase and involving glycolysis, TCA and glyoxylate cycles of carbon metabolic processes were higher expressed in the DRB treatment when compared with the ECO. Nevertheless, the genes for glutathione metabolism were more upregulated in the control than those in both bacterial treatments, regardless of the digestibility of the bacteria. The results of this study indicate that not only bacterial food but also digestibility of bacterial taxa modulate multiple metabolic processes in heterotrophic protists, which contribute to a better understanding of protistan bacterivory and bacteria-protists interactions on a molecular basis.
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DOI：
--
发表时间：
1977
期刊：
Acta biologica et medica Germanica
影响因子：
--
作者：
H. Kirschke;J. Langner;B. Wiederanders;S. Ansorge;P. Bohley;H. Hanson
通讯作者：
H. Kirschke;J. Langner;B. Wiederanders;S. Ansorge;P. Bohley;H. Hanson
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
56.9
作者：
HWANG, C;SINSKEY, AJ;LODISH, HF
通讯作者：
LODISH, HF
DOI：
10.1016/s0140-6736(80)91745-6
发表时间：
1980-06
期刊：
The Federal Theology of Johannes Cocceius (1603-1669)
影响因子：
--
作者：
George Vogiatzis;Carlos Hernández;R. Cipolla
通讯作者：
George Vogiatzis;Carlos Hernández;R. Cipolla
影响因子：
4.4
作者：
Lie AA;Liu Z;Terrado R;Tatters AO;Heidelberg KB;Caron DA
通讯作者：
Caron DA