A conserved family of WD-40 proteins binds to the retinoblastoma protein in both plants and animals
A conserved family of WD-40 proteins binds to the retinoblastoma protein in both plants and animals
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DOI：
10.1105/tpc.9.9.1595
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发表时间：
1997-09-01
期刊：
影响因子：
11.6
通讯作者：
Gruissem, W
中科院分区：
文献类型：
--
作者：
Ach, RA;Taranto, P;Gruissem, W
在哺乳动物细胞中，视网膜母细胞瘤（RB）蛋白通过与各种细胞蛋白的结合来调节G（1）进程和功能。两种密切相关的哺乳动物RB结合蛋白RbAp 48和RbAp 46与酵母的Msi 1蛋白共享序列同源性。MSI 1是IRA 1基因突变的多拷贝抑制因子，该基因参与调节细胞生长的Ras-cAMP途径。人RbAp 48存在于参与组蛋白乙酰化和染色质组装的蛋白质复合物中。我们报告的cDNA编码四个植物RbAp 48和Msi 1样蛋白的克隆：一个从番茄，LeMSI 1，和三个从拟南芥。互补研究证实，LeMSI 1可以作为酵母IRA 1突变表型的多拷贝抑制因子。LeMSI 1蛋白定位于细胞核，并与野生型以及成熟抑制剂（rin）和Neverripe（Nr）番茄果实中的65 kD蛋白结合。LeMSI 1还与人RB蛋白和来自玉米的RB样RRB 1蛋白结合，表明这种相互作用在植物和动物之间是保守的。
In mammalian cells, the retinoblastoma (RB) protein regulates G(1) progression and functions through its association with various cellular proteins. Two closely related mammalian RB binding proteins, RbAp48 and RbAp46, share sequence homology with the Msi1 protein of yeast. MSI1 is a multicopy suppressor of a mutation in the IRA1 gene involved in the Ras-cAMP pathway that regulates cellular growth. Human RbAp48 is present in protein complexes involved in histone acetylation and chromatin assembly. We report the cloning of cDNAs encoding four plant RbAp48- and Msi1-like proteins: one from tomato, LeMSI1, and three from Arabidopsis. Complementation studies confirm that LeMSI1 can function as a multicopy suppressor of the yeast ira1 mutant phenotype. The LeMSI1 protein localizes to the nucleus and binds to a 65-kD protein in wild-type as well as ripening inhibitor (rin) and Neverripe (Nr) tomato fruit. LeMSI1 also binds to the human RB protein and the RB-like RRB1 protein from maize, indicating that this interaction is conserved between plants and animals.