Effects of basic fibroblast growth factor administration on vascular changes in wound healing of rat palates
Effects of basic fibroblast growth factor administration on vascular changes in wound healing of rat palates
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DOI：
10.1597/06-166.1
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发表时间：
2008-01-01
期刊：
影响因子：
--
通讯作者：
Ishikawa, Hiroyuki
中科院分区：
文献类型：
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作者：
Hata, Yuichiro;Kawanabe, Hitoshi;Ishikawa, Hiroyuki
目的：观察大鼠腭粘膜骨膜剥落后血管的变化，探讨碱性成纤维细胞生长因子（bFGF）对腭血管网络的影响。方法：选取雄性Wistar大鼠117只，于其出生后第20天进行实验。这些动物被分为三组：疤痕形成组、基本成纤维细胞生长因子组和对照组。瘢痕形成组和碱性成纤维细胞生长因子组均切除腭侧粘骨膜。碱性成纤维细胞生长因子组，术后1周注射碱性成纤维细胞生长因子溶液。在术后6、8和10周，通过免疫组织化学染色和腐蚀铸造技术研究腭血管的变化。结果：在整个实验期间，瘢痕形成组血管数量明显少于对照组和碱性成纤维细胞生长因子组。在碱性成纤维细胞生长因子组，新血管的延伸和毛细血管的增殖继续进行，10周后，一个高度组织的血管网络建立起来。瘢痕形成组显示很少的Volkmann管萎缩或关闭，而碱性成纤维细胞生长因子组显示Volkmann管有小动脉或小静脉，如对照组所见。结论：在腭创面内注入碱性成纤维细胞生长因子，可改善腭创面愈合过程中及愈合后对手术黏膜和下底骨的血管供应，促进腭生长过程中的组织重塑。
Objective: The purpose of this study was to investigate the vascular changes induced by mucoperiosteal denudation of rat palate and to elucidate the effects of basic fibroblast growth factor (bFGF) administration on the palatal vascular network in wound healing.Methods: A total of 117 male Wistar rats were used for the study on their 20th postnatal day. The animals were divided into three groups: a scar formation group, a basic fibroblast growth factor group, and a control group. The scar formation and basic fibroblast growth factor groups had lateral mucoperiosteum excised from the palate. In the basic fibroblast growth factor group, a solution of basic fibroblast growth factor was injected into the operated area 1 week after excision. At 6, 8, and 10 weeks postoperatively, palatal vascular changes were investigated by immunohistochemical staining and corrosion cast techniques.Results: Throughout the experimental period, there were significantly fewer vessels in the scar formation group than in the control and basic fibroblast growth factor groups. In the basic fibroblast growth factor group, the elongation of new vessels and capillary proliferation proceeded, and after 10 weeks a highly organized vascular network was established. The scar formation group showed few Volkmann's canals that were shrunken or closed, whereas the basic fibroblast growth factor group evidenced Volkmann's canals with arterioles or venules, as seen in the control.Conclusions: The results suggested that injection of basic fibroblast growth factor into palatal wounds improves the vascular supply to the operated mucosa and underlying bone during and after palatal wound healing, which may contribute to tissue remodeling of the palate during growth.