PLATELETS AS A SOURCE OF FIBROBLAST GROWTH-PROMOTING ACTIVITY
PLATELETS AS A SOURCE OF FIBROBLAST GROWTH-PROMOTING ACTIVITY
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DOI：
10.1016/0014-4827(74)90484-4
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发表时间：
1974-01-01
影响因子：
3.7
通讯作者：
LIPTON, A
中科院分区：
文献类型：
--
作者：
KOHLER, N;LIPTON, A
有证据表明，血小板是一个丰富的来源，3 T3细胞，小鼠成纤维细胞系的生长促进活性。大鼠或人血清比自体血浆更能刺激3 T3细胞生长;这种增加的活性至少部分是由于凝血过程中破裂的血小板释放出促生长活性。血小板提取物对3 T3细胞生长的刺激约为比等量自体血清高5倍。这种血小板生长活性是（1）pH稳定的;（2）胃蛋白酶不稳定的;（3）在56 °C下热稳定的;（4）不可透析的，以及（5）在大小上与血清3 T3生长促进分子相似。猴病毒40转化的3 T3细胞显示出与血小板溶液和血清相当的刺激作用。
Evidence is presented that platelets are an enriched source of growth-promoting activity for 3T3 cells, an established line of mouse fibroblasts. Rat or human serum stimulates 3T3 cell growth more than autologous plasma; this increased activity is at least partly due to the release of a growth-promoting activity from platelets disrupted during the clotting process. Platelet extract stimulation of 3T3 cell growth is approx. 5 times greater than an equal amount of autologous serum. This platelet growth activity is (1) pH-stable; (2) pepsin-labile; (3) heat-stable at 56 °C; (4) non-dialysable, and (5) similar in size to the serum 3T3 growth-promoting molecule. 3T3 cells transformed by Simian virus 40 show an equivalent stimulation by the platelet solution and serum.