Culture of human umbilical vein endothelial cells on immobilized vascular endothelial growth factor
Culture of human umbilical vein endothelial cells on immobilized vascular endothelial growth factor
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DOI：
10.1002/jbm.a.30360
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发表时间：
2005-09-15
影响因子：
4.9
通讯作者：
Kitajima, T
中科院分区：
文献类型：
--
作者：
Ito, Y;Hasuda, H;Kitajima, T
将血管内皮生长因子（VEGF）固定在光反应明胶基质上，以控制血管内皮细胞的粘附和生长。将明胶和VEGF在水中混合并浇铸在聚苯乙烯皿或硅烷涂覆的玻璃板上。然后在存在或不存在光掩模的情况下对表面进行光照射并洗涤。通过乙醇处理证实了固定化材料的韧性。人脐静脉内皮细胞（HUVECs）生长在固定的VEGF，但不是在未经处理的表面。HUVEC的生长随着固定VEGF量的增加而显著增加，并且这种作用被抗VEGF抗体抑制。因此，固定的VEGF特异性地与HUVEC相互作用以允许在培养物中生长。HUVEC培养物的微图案化也使用微图案固定的VEGF实现。这种图案化技术可用于体外血管网络的形成。(c)2005 Wiley Periodicals，Inc.
Vascular endothelial growth factor (VEGF) was immobilized on substrata in photoreactive gelatin to control the adhesion and growth of vascular endothelial cells. The gelatin and VEGF were mixed in water and cast on a polystyrene dish or a silane-coated glass plate. The surface was then photoirradiated in the presence or absence of a photomask and washed. Toughness of the immobilized material was confirmed by ethanol treatment. Human umbilical vein endothelial cells (HUVECs) grew on the immobilized VEGF but not on a nontreated surface. Growth of HUVEC increased significantly with an increase in the amount of immobilized VEGF, and the effects were inhibited by treatment with anti-VEGF antibody. Thus, immobilized VEGF specifically interacted with HUVECs to permit growth in culture. Micropatterning of HUVEC cultures was also achieved using micropattern-immobilized VEGF. This patterning technique may be useful for the formation of blood vessel networks iii vitro. (c) 2005 Wiley Periodicals, Inc.