Design of an artificial light-harvesting unit by protein engineering: cytochrome b(562)-green fluorescent Protein chimera.
Design of an artificial light-harvesting unit by protein engineering: cytochrome b(562)-green fluorescent Protein chimera.
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通过蛋白质工程设计人工光采集单元：细胞色素 b(562)-绿色荧光蛋白嵌合体。
DOI：
10.1006/bbrc.2001.5966
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发表时间：
2001
影响因子：
3.1
通讯作者：
T. Nagamune
中科院分区：
文献类型：
--
作者：
S. Takeda;N. Kamiya;R. Arai;T. Nagamune
我们已经为一种由细胞色素b(562) （cytb(562)）和增强型绿色荧光蛋白（EGFP）两种蛋白质组成的人工光收集复合物生成了一种新的模型蛋白，其中两个发色团固定在每个蛋白质基质中。通过Gly-Ser连接器将Cytb（562）连接到EGFP的n端，并在大肠杆菌中成功表达了Cytb（562）部分的载子和全型混合融合蛋白。用血红蛋白重组载脂蛋白后，EGFP的荧光基本被猝灭。根据荧光寿命测量，EGFP捕获的光能通过共振能量转移（能量转移率为65%）转移到cytb（562）的血红素上。利用小而稳定的蛋白质基质对两种发色团进行空间组织，有望通过蛋白质工程构建人工光收集复合物。
We have generated a novel model protein for an artificial light-harvesting complex composed of two proteins, cytochrome b(562) (cytb(562)) and enhanced green fluorescent protein (EGFP), in which two chromophores are fixed in each protein matrix. Cytb(562) was appended to the N-terminus of EGFP via a Gly-Ser linker and the resultant fusion protein was successfully expressed in Escherichia coli as a mixture of the apo- and the holo-forms as to the cytb(562) moiety. The fluorescence of EGFP was substantially quenched when the apo-form was reconstituted with hemin. Based on the fluorescence lifetime measurements, it appeared that light energy entrapped by EGFP is transferred to the heme of cytb(562) by resonance energy transfer (energy transfer yield: 65%). Spatial organization of two chromophores using small and stable protein matrices will be promising toward the construction of an artificial light-harvesting complex by protein engineering.
编码大肠杆菌可溶性细胞色素b562的基因的克隆和表达。
DOI：
10.1111/j.1432-1033.1991.tb16377.x
发表时间：
1991
期刊：
European journal of biochemistry
影响因子：
--
作者：
Nikkila,H;Gennis,RB;Sligar,SG
通讯作者：
Sligar,SG
细胞色素 b562 变体：用于检查氧化还原电位演化的库。
影响因子：
2.9
作者：
Springs,SL;Bass,SE;McLendon,GL
通讯作者：
McLendon,GL