Evidence of temporal stability in allelic and mitochondrial haplotype diversity in populations of Glossina fuscipes fuscipes (Diptera: Glossinidae) in northern Uganda.
Evidence of temporal stability in allelic and mitochondrial haplotype diversity in populations of Glossina fuscipes fuscipes (Diptera: Glossinidae) in northern Uganda.
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乌干达北部的Glossina fuscipes fuscipes（双翅目：Glossinidae）种群中等位基因和线粒体单倍型多样性的时间稳定的证据。
DOI：
10.1186/s13071-016-1522-5
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发表时间：
2016-05-03
影响因子：
3.2
通讯作者：
Caccone A
中科院分区：
文献类型：
--
作者：
Opiro R;Saarman NP;Echodu R;Opiyo EA;Dion K;Halyard A;Aksoy S;Caccone A
fuscipes fuscipes是乌干达一种具有高度经济重要性的采采蝇，它负责传播动物非洲锥虫病（AAT）以及慢性和急性非洲人类锥虫病（HAT）。我们使用来自17颗微卫星的基因型数据和线粒体DNA标记来评估2008年至2014年期间在乌干达北部已知存在两种形式HAT的9个地区收集的样本的基因频率的时间变化。我们的研究结果表明，在采样的时间跨度内，两个HAT疫源地的大多数研究种群在遗传上是稳定的。使用标准化FST和Jost’s DEST在每个地点采样的时间点之间的两两分化估计通常较低，两组指数的范围在0.0019和0.1312之间。我们观察到Kitgum (KT), Karuma (KR), Moyo （MY）和Pader （PD）采样的时间点之间的FST和DEST值最高，并讨论了可能的原因。使用Waple时间法估计的有效种群规模（Ne）范围从Kitgum的103 （95% CI: 73-138）到Oculoi （OC）的962 （95% CI: 669-1309）。此外，在一个采样时间点，仅在一个种群中检测到瓶颈事件的证据；Aminakwach (AM-27)， 2014年12月（P < 0.03889）。研究结果表明，在乌干达北部两种形式的HAT疫源地，采采媒介总体上具有时间稳定性。遗传稳定性和适度的有效种群规模意味着媒介从控制努力错过的当地残余种群中重新出现是一个重要的风险。这强调需要更灵敏的抽样和监测，以便在控制活动后发现残留种群。
Glossina fuscipes fuscipes is a tsetse species of high economic importance in Uganda where it is responsible for transmitting animal African trypanosomiasis (AAT) and both the chronic and acute forms of human African trypanosomiasis (HAT). We used genotype data from 17 microsatellites and a mitochondrial DNA marker to assess temporal changes in gene frequency for samples collected between the periods ranging from 2008 to 2014 in nine localities spanning regions known to harbor the two forms of HAT in northern Uganda. Our findings suggest that the majority of the studied populations in both HAT foci are genetically stable across the time span sampled. Pairwise estimates of differentiation using standardized FST and Jost’s DEST between time points sampled for each site were generally low and ranged between 0.0019 and 0.1312 for both sets of indices. We observed the highest values of FST and DEST between time points sampled from Kitgum (KT), Karuma (KR), Moyo (MY) and Pader (PD), and the possible reasons for this are discussed. Effective population size (Ne) estimates using Waple’s temporal method ranged from 103 (95 % CI: 73–138) in Kitgum to 962 (95 % CI: 669–1309) in Oculoi (OC). Additionally, evidence of a bottleneck event was detected in only one population at one time point sampled; Aminakwach (AM-27) from December 2014 (P < 0.03889). Findings suggest general temporal stability of tsetse vectors in foci of both forms of HAT in northern Uganda. Genetic stability and the moderate effective population sizes imply that a re-emergence of vectors from local residual populations missed by control efforts is an important risk. This underscores the need for more sensitive sampling and monitoring to detect residual populations following control activities.