Photodynamic activation as a molecular switch to promote osteoblast cell differentiation via AP-1 activation.
Photodynamic activation as a molecular switch to promote osteoblast cell differentiation via AP-1 activation.
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DOI：
10.1038/srep13114
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发表时间：
2015-08-17
影响因子：
4.6
通讯作者：
Hasan T
中科院分区：
文献类型：
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作者：
Kushibiki T;Tu Y;Abu-Yousif AO;Hasan T
在光动力疗法（PDT）中，细胞浸渍有通过光照射激活的光敏剂，从而光化学地产生活性氧物质（ROS）。产生的ROS的量取决于PDT剂量和光敏剂的性质。虽然高水平的ROS具有细胞毒性，但在生理水平上，它们作为第二信使在细胞信号传导途径、多能性和干细胞分化中发挥关键作用。为了进一步研究使用光化学触发操纵这些途径，我们暴露小鼠成骨细胞前体细胞和大鼠原代间充质基质细胞的低剂量PDT。我们的研究结果表明，低剂量PDT可以通过激活激活蛋白-1（AP-1）促进成骨细胞分化。虽然PDT主要用于抗癌治疗，但使用光作为光化学“分子开关”来促进分化应扩大该方法在基础研究和临床应用中的实用性。
In photodynamic therapy (PDT), cells are impregnated with a photosensitizing agent that is activated by light irradiation, thereby photochemically generating reactive oxygen species (ROS). The amounts of ROS produced depends on the PDT dose and the nature of the photosensitizer. Although high levels of ROS are cytotoxic, at physiological levels they play a key role as second messengers in cellular signaling pathways, pluripotency, and differentiation of stem cells. To investigate further the use of photochemically triggered manipulation of such pathways, we exposed mouse osteoblast precursor cells and rat primary mesenchymal stromal cells to low-dose PDT. Our results demonstrate that low-dose PDT can promote osteoblast differentiation via the activation of activator protein-1 (AP-1). Although PDT has been used primarily as an anti-cancer therapy, the use of light as a photochemical “molecular switch” to promote differentiation should expand the utility of this method in basic research and clinical applications.