Transcriptome Analysis of Two Strains of Proteus mirabilis with Swarming Migration Deficiency Isolated from Patients with Urinary Tract Infection
Transcriptome Analysis of Two Strains of Proteus mirabilis with Swarming Migration Deficiency Isolated from Patients with Urinary Tract Infection
复制标题
尿路感染患者分离的两株具有群迁移缺陷的奇异变形杆菌的转录组分析
DOI：
10.1007/s00284-020-01931-6
复制
发表时间：
2020-03
影响因子：
2.6
通讯作者：
Sun Ning
中科院分区：
文献类型：
--
作者：
Peng Liang;Chen Ding-Qiang;Jiang Guan-Ming;Ou Jing-Yi;Jiang Qiao;Zeng Li-Ting;Xiao Yi;Jiang Qiong-Yan;Yang Ling;Sun Ning
从2例尿路感染患者的尿液中分离到2株罕见的群体迁移缺陷变形杆菌，分别命名为G121和G137。迁移实验表明，奇异P.mirabilis HI4320在血液琼脂上具有典型的迁移能力，而G121和G137迁移能力明显减弱。黏附实验结果显示，G121和G137对膀胱上皮细胞株5637的黏附能力明显降低。以奇异拟青霉HI4320为参比菌株，对3株奇异拟青霉的转录本进行了高通量测序和比对分析。用逆转录定量聚合酶链式反应(RT-qPCR)验证差异表达基因。转录组分析和RT-qPCR结果表明，与HI4320菌株相比，G121和G137中与鞭毛和菌毛形成、二羧酸转运、胱硫醚和邻氨基苯甲酸代谢相关的基因表达下调，而与铁运输、钼代谢和金属蛋白酶相关的基因表达上调，提示这些基因可能与奇异P.mirabilis的迁移能力和上皮细胞的黏附能力有关。这些结果为寻找奇异假单胞菌的毒力基因和筛选新的抗菌靶点提供了重要的启示。
Two rare strains of Proteus mirabilis with swarming migration deficiency were isolated from urine samples of two patients with urinary tract infections and were named as G121 and G137. Migration experiments showed that P. mirabilis HI4320 had typical migration on blood agar, while G121 and G137 had significantly weakened migration ability. Results of adhesion tests showed that the adhesion ability of G121 and G137 to the bladder epithelial cell line 5637 was significantly reduced. High-throughput sequencing and alignment analysis of the transcriptomes of the three P. mirabilis strains were conducted, with P. mirabilis HI4320 as the reference strain. Reverse transcription quantitative PCR (RT-qPCR) was used to verify differentially expressed genes. Results of transcriptome analysis and RT-qPCR showed that, compared to the HI4320 strain, genes related to flagellum and fimbria formation, dicarboxylate transport, and cystathionine and anthranilate metabolism were down-regulated in G121 and G137, while genes related to iron transport, molybdenum metabolism, and metalloprotease were up-regulated, suggesting that these genes may be involved in the migration ability and epithelial cell adhesion ability of P. mirabilis. These results provide important insight to the search for virulence genes and the screening of new antibacterial targets for P. mirabilis.
登录
查看更多内容
DOI：
10.1007/978-1-4471-5547-8_35
发表时间：
2014
期刊：
--
影响因子：
--
作者：
G. Rajakaruna;M. Harber
通讯作者：
G. Rajakaruna;M. Harber
影响因子：
3.1
作者：
S. K. Wray;S. Hull;R. Cook;J. Barrish;R. Hull
通讯作者：
S. K. Wray;S. Hull;R. Cook;J. Barrish;R. Hull
影响因子：
--
作者：
Chi F;Wang Y;Gallaher TK;Wu CH;Jong A;Huang SH
通讯作者：
Huang SH
DOI：
10.3109/9780203091456-8
发表时间：
2008-05
期刊：
--
影响因子：
--
作者：
Stuart J O’Toole
通讯作者：
Stuart J O’Toole
DOI：
10.12703/p7-14
发表时间：
2015
期刊：
F1000prime reports
影响因子：
--
作者：
Wilkens S
通讯作者：
Wilkens S