Tuning of Titanium Microfiber Scaffold with UV-Photofunctionalization for Enhanced Osteoblast Affinity and Function
Tuning of Titanium Microfiber Scaffold with UV-Photofunctionalization for Enhanced Osteoblast Affinity and Function
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DOI：
10.3390/ijms21030738
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发表时间：
2020-02-01
影响因子：
5.6
通讯作者：
Ogawa, Takahiro
中科院分区：
文献类型：
--
作者：
Iwasaki, Chika;Hirota, Makoto;Ogawa, Takahiro
钛（Ti）是一种骨传导材料，通常用作大块植入物，以固定和修复骨骼和牙齿。本研究旨在探索钛作为骨工程支架材料的有效应用。需要克服的挑战是：（1）由于Ti的疏水性，液体/细胞难以渗透到Ti微纤维支架中;和（2）细胞难以附着在薄且弯曲的Ti微纤维上。最近发现的紫外光功能化的钛促使我们检查其对钛微纤维支架的影响。通过编织4级纯钛微纤维（直径125 μ m）制成圆盘形支架，并对其中一半进行酸蚀刻以使表面粗糙化。在细胞培养之前，一些具有原始或酸蚀刻表面的支架进一步通过UV光处理。钛微纤维支架，无论表面类型，是疏水性的，不允许甘油/水液体渗透，而在UV处理后，支架变成亲水性的，并立即吸收液体。两种不同来源的成骨细胞，分别来自大鼠股骨和下颌骨骨髓，在支架上培养。经紫外线处理的支架在培养早期24 h内贴壁细胞数增加3-10倍。细胞质突起和细胞骨架的发展，以及黏着斑蛋白的表达，只观察到UV处理的支架。成骨细胞的功能表型，如碱性磷酸酶活性和钙矿化，在紫外线处理的支架上是2-15倍，与原始支架相比，在酸蚀刻的支架上有更明显的增强。紫外线处理的这些影响与钛微纤维表面原子碳的显着减少有关。紫外线处理钛微纤维支架可调节其理化性能，有效增强成骨细胞的附着和功能，为骨工程提供了新的策略。
Titanium (Ti) is an osteoconductive material that is routinely used as a bulk implant to fix and restore bones and teeth. This study explored the effective use of Ti as a bone engineering scaffold. Challenges to overcome were: (1) difficult liquid/cell infiltration into Ti microfiber scaffolds due to the hydrophobic nature of Ti; and (2) difficult cell attachment on thin and curved Ti microfibers. A recent discovery of UV-photofunctionalization of Ti prompted us to examine its effect on Ti microfiber scaffolds. Scaffolds in disk form were made by weaving grade 4 pure Ti microfibers (125 mu m diameter) and half of them were acid-etched to roughen the surface. Some of the scaffolds with original or acid-etched surfaces were further treated by UV light before cell culture. Ti microfiber scaffolds, regardless of the surface type, were hydrophobic and did not allow glycerol/water liquid to infiltrate, whereas, after UV treatment, the scaffolds became hydrophilic and immediately absorbed the liquid. Osteogenic cells from two different origins, derived from the femoral and mandibular bone marrow of rats, were cultured on the scaffolds. The number of cells attached to scaffolds during the early stage of culture within 24 h was 3-10 times greater when the scaffolds were treated with UV. The development of cytoplasmic projections and cytoskeletal, as well as the expression of focal adhesion protein, were exclusively observed on UV-treated scaffolds. Osteoblastic functional phenotypes, such as alkaline phosphatase activity and calcium mineralization, were 2-15 times greater on UV-treated scaffolds, with more pronounced enhancement on acid-etched scaffolds compared to that on the original scaffolds. These effects of UV treatment were associated with a significant reduction in atomic carbon on the Ti microfiber surfaces. In conclusion, UV treatment of Ti microfiber scaffolds tunes their physicochemical properties and effectively enhances the attachment and function of osteoblasts, proposing a new strategy for bone engineering.