Role of conserved cysteines in mediating sulfur transfer from IscS to IscU.
Role of conserved cysteines in mediating sulfur transfer from IscS to IscU.
复制标题
保守半胱氨酸在介导硫从 IscS 转移到 IscU 中的作用。
DOI：
10.1016/j.febslet.2005.08.046
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Johnson,MichaelK
中科院分区：
文献类型：
--
作者：
Smith,ArcherD;Frazzon,Jeverson;Dean,DennisR;Johnson,MichaelK
用电喷雾电离质谱法研究了棕色固氮菌IscU上三个保守的半胱氨酸残基在接受硫磺和与ISCS形成共价复合体中的作用。结果表明，ISCS可以将硫转移到ISCU的三种丙氨酸取代形式中，生成过硫化物或多硫化物物种，并与ISCU上的Cys37形成异二硫键共价络合物。结论：S从Iscs到Iscu的转移不涉及IscU上的特定半胱氨酸，也不涉及Iscs-Iscu杂二硫化物的形成。
The role of the three conserved cysteine residues on Azotobacter vinelandii IscU in accepting sulfane sulfur and forming a covalent complex with IscS has been evaluated using electrospray-ionization mass spectrometry studies of variants involving individual cysteine-to-alanine substitutions. The results reveal that IscS can transfer sulfur to each of the three alanine-substituted forms of IscU to yield persulfide or polysulfide species, and formation of a heterodisulfide covalent complex between IscS and Cys37on IscU. It is concluded that S transfer from IscS to IscU does not involve a specific cysteine on IscU or the formation of an IscS–IscU heterodisulfide complex.