Four-dimensional analysis of nucleogenesis of the pontine nucleus in the hindbrain
Four-dimensional analysis of nucleogenesis of the pontine nucleus in the hindbrain
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后脑脑桥核核发生的四维分析
DOI：
10.1002/cne.23353
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Fujio Murakami
中科院分区：
文献类型：
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作者：
Masaki Shinohara;Yan Zhu;Fujio Murakami
中枢神经系统的细胞核是神经元的三维聚集体，具有共同的生理特性、功能和连通性。为了形成特定的细胞核，神经元从它们的诞生地迁移到假定的核区域，在那里它们改变它们的动态，聚集并重新排列成一个独特的3D结构，这个过程我们称之为核发生。细胞核与层状结构一起构成了处理信息的基本细胞结构单元。然而，与研究较多的层流结构相反，有助于聚集过程形成细胞核的神经元动力学知之甚少。在这里，我们通过观察小鼠小脑前桥脑核（PN）来分析核发生，并通过沿着三个空间轴随时间跟踪神经元行为提供核发生的第一个4D视图。早期和晚期出生的PN神经元通过子宫内电穿孔进行标记，并通过延时成像记录其在培养脑切片上的行为。我们发现，当PN神经元向内迁移到核区时，其中许多神经元切换为径向和侧向迁移，分别填充背侧和外侧PN区。在晚出生的神经元中，转向径向迁移的倾向要少得多，而转向横向迁移的倾向在两组之间是相当的。与径向和中外侧轴相反，很少有PN神经元切换到倒向迁移。因此，这些结果可以为理解调节这一复杂而重要的过程的机制提供一个框架。中国生物医学工程学报，2013,31(2):444 - 444。©2013 Wiley期刊公司
Nuclei in the central nervous system are 3D aggregates of neurons that have common physiological properties, functionalities, and connectivities. To form specific nuclei, neurons migrate from their birthplace towards the presumptive nuclear region where they change their dynamics to aggregate and rearrange into a distinct 3D structure, a process that we term nucleogenesis. Nuclei, together with the laminar structure, form the basic cytoarchitectonic unit for information processing. However, in contrast to much‐studied laminar structures, the neuronal dynamics that contribute to the aggregation process to form nuclei are poorly understood. Here, we analyze nucleogenesis by observing the mouse precerebellar pontine nucleus (PN), and provide the first 4D view of nucleogenesis by tracking neuronal behaviors along the three spatial axes over time. Early‐ and late‐born PN neurons were labeled by in utero electroporation and their behaviors on cultured brain slices were recorded by time‐lapse imaging. We find that when PN neurons migrate medially into the nuclear region, many of them switch to migrate radially and laterally, to populate the dorsal and lateral PN regions, respectively. The tendency to switch to radial migration is much less in later‐born neurons, whereas that to switch to lateral migration is comparable between the two groups. In contrast to the radial and mediolateral axes, very few PN neurons switch to migrate rostrocaudally. These results could thus provide a framework for understanding the mechanisms that regulate this complex yet important process. J. Comp. Neurol. 521:3340–3357, 2013. © 2013 Wiley Periodicals, Inc.