Highly activatable and rapidly releasable caged fluorescein derivatives
Highly activatable and rapidly releasable caged fluorescein derivatives
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DOI：
10.1021/ja070376d
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发表时间：
2007-05-30
影响因子：
15
通讯作者：
Nagano, Tetsuo
中科院分区：
文献类型：
--
作者：
Kobayashi, Tomonori;Urano, Yasuteru;Nagano, Tetsuo
笼状荧光团在紫外光照射下恢复荧光活性，在研究细胞谱系和细胞蛋白质动力学方面发挥了重要作用。在本文中，我们报告了新的硝基苄基笼状荧光素衍生物，基于我们的TokyoGreen平台（笼状TokyoGreen），它有一个单一的光可去除的保护基团，荧光量子效率可以通过光诱导的电子转移过程进行精细控制之前和之后的光活化。这些新的衍生物可以被更迅速地激活，并显示出更大的荧光增强比传统的笼状荧光素，这是一个双笼内酯的形式，在短暂的紫外线照射在生物应用和比色皿实验。
Caged fluorophores, which recover fluorescence activity upon brief illumination with ultraviolet (UV) light, have played an important role in investigating cell lineage and cellular protein dynamics. In this paper, we report novel nitrobenzyl-caged fluorescein derivatives, based on our TokyoGreen platform (caged TokyoGreen), which have a single photoremovable protecting group, and the fluorescence quantum efficiency can be finely controlled via the photoinduced electron-transfer process before and after photoactivation. These new derivatives can be activated more rapidly and show a larger fluorescence enhancement than a traditional caged fluorescein, which is a bis-caged lactone form, upon brief UV irradiation both in the biological application and in cuvette experiments.