Transcription of the caveolin-1 gene is differentially regulated in lung type I epithelial and endothelial cell lines - A role for ETS proteins in epithelial cell expression
Transcription of the caveolin-1 gene is differentially regulated in lung type I epithelial and endothelial cell lines - A role for ETS proteins in epithelial cell expression
复制标题
DOI：
10.1074/jbc.m402236200
复制
发表时间：
2004-07-16
影响因子：
4.8
通讯作者：
Williams, MC
中科院分区：
文献类型：
--
作者：
Kathuria, H;Cao, YX;Williams, MC
在肺中，小窝蛋白-1在I型肺泡上皮细胞和内皮细胞中表达，假设其调节分子信号传导活性和肿瘤发生的进展。在发育过程中，小窝蛋白-1 α在胎儿肺内皮细胞中表达，但在上皮细胞中不表达;在成人肺中，两种细胞类型都表达小窝蛋白-1 α。为了验证小窝蛋白-1转录在I型细胞和内皮细胞中受到差异调节的假设，我们表征了肺细胞系中小鼠小窝蛋白-1基因的近端启动子，以确定控制其细胞特异性表达的因素。我们发现，小窝蛋白-1的表达是由一个Ets顺式元件在肺上皮细胞系，但不是肺内皮细胞系，和三个ETS家族成员，ETS-1，PEA 3，和ERM，识别和结合的Ets网站在上皮细胞系。基于这些发现，我们已经确定了Ets顺式元件作为一个区域，占差异转录调节小窝蛋白-1在肺上皮细胞和内皮细胞。
In the lung, caveolin-1 is expressed in both type I alveolar epithelial and endothelial cells where it is hypothesized to modulate molecular signaling activities and progression of tumorigenesis. Developmentally, caveolin-1alpha is expressed in fetal lung endothelial, but not epithelial, cells; in adult lung, both cell types express caveolin-1alpha. To test the hypothesis that caveolin-1 transcription is differentially regulated in type I and endothelial cells, we characterized the proximal promoter of the mouse caveolin-1 gene in lung cell lines to identify factors that control its cell-specific expression. We show that caveolin-1 expression is regulated by an Ets cis-element in a lung epithelial cell line, but not a lung endothelial cell line, and that three ETS family members, ETS-1, PEA3, and ERM, recognize and bind the Ets site in the epithelial cell line. Based on these findings, we have identified the Ets cis-element as a region that accounts for differential transcriptional regulation of caveolin-1 in lung epithelial and endothelial cells.