Use of FOXJ1CreER2T mice for inducible deletion of embryonic node gene expression.
Use of FOXJ1CreER2T mice for inducible deletion of embryonic node gene expression.
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使用 FOXJ1CreER2T 小鼠诱导胚胎节点基因表达缺失。
DOI：
10.1002/dvg.20467
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Ware,StephanieM
中科院分区：
文献类型：
--
作者：
Wang,Shuyun;Ware,StephanieM
The ciliated cells of the node of the mouse embryo contribute to the establishment of left–right patterning via generation of leftward laminar fluid flow and initiation of a left‐sided morphogen gradient. Here, we identifyFOXJ1CreER2Tmice in which expression of Cre recombinase is directed to ciliated node cells. The data demonstrate thatfoxj1is expressed specifically in the node throughout the developmental window critical for left–right patterning. In transgenic embryos, Cre expression is detected by immunohistochemistry in ciliated cells of the node.Rosa26Rreporter mice, in which expression oflacZis activated only after Cre‐mediated recombination, demonstrate strong and uniform labeling at the node when crossed withFOXJ1CreER2Tmice. Cell labeling occurred as early as 0‐ to 2‐somite stages, specifically within cells of the node, and recombination was highly efficient in response to tamoxifen.FOXJ1CreER2Ttransgenic mice represent a new genetic tool for the analysis of node‐specific gene expression and will also be valuable in the study of node cell lineage and temporal cell fate mapping. genesis 47:132–136, 2009. © 2009 Wiley‐Liss, Inc.
