FORMATION OF OSTEOBLAST-LIKE CELLS FROM HUMAN MONONUCLEAR BONE-MARROW CULTURES
FORMATION OF OSTEOBLAST-LIKE CELLS FROM HUMAN MONONUCLEAR BONE-MARROW CULTURES
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DOI：
10.1111/j.1699-0463.1991.tb05149.x
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发表时间：
1991-03-01
期刊：
影响因子：
2.8
通讯作者：
ERIKSEN, EF
中科院分区：
文献类型：
--
作者：
KASSEM, M;RISTELI, L;ERIKSEN, EF
成骨细胞样细胞通常存在于长期培养的人骨髓中形成的破骨细胞附近，它们被认为来源于属于基质细胞系统的成骨细胞前体。本文介绍了一种利用长期培养的人单核骨髓贴壁细胞群培养人成骨细胞的新方法。获得的细胞表现出成骨细胞的所有典型特征。它们含有高浓度的细胞内碱性磷酸酶，并分泌成骨细胞特异性标记物骨玻璃蛋白。在条件培养基中，产生的胶原主要为I型前胶原前肽（95% -98%），放射免疫法仅检测到微量的III型前胶原前肽。8周后，细胞暴露于-甘油磷酸酯和抗坏血酸中形成矿化基质。该系统为体外研究成骨细胞分化提供了一个模型，并且由于存在高浓度的成骨细胞前体，可能形成在骨细胞培养中使用定义培养基的基础。
Osteoblast-like cells are commonly found in the vicinity of osteoclasts formed in long-term human bone marrow cultures, and they are believed to be derived from osteogenic cell precursors belonging to the stromal cell system. This paper describes a new culture method for human osteoblasts from the adherent cell population of long-term human mononuclear bone marrow cultures. The cells obtained exhibited all the classic characteristics of osteoblasts. They contained high intracellular concentrations of alkaline phosphatase and they secreted the osteoblast-specific marker bone Gla protein. Collagen production was mainly (95-98%) procollagen type I propeptide and only minute quantities of procollagen type III propeptide were detectable by radioimmunoassay in the conditioned medium. After eight weeks the cells formed a mineralized matrix on exposure to beta-glycerophosphate and ascorbic acid. This system provides a model for the study of osteoblast differentiation in vitro and may form the basis for the use of defined media in bone cell cultures due to the presence of high concentrations of osteoblast precursors.