PARTIAL CHARACTERIZATION OF PRINCIPAL SOLUBLE-ANTIGENS ASSOCIATED WITH CORONAVIRUS OF TRANSMISSIBLE GASTROENTERITIS BY COMPLEMENT-FIXATION AND IMMUNODIFFUSION
PARTIAL CHARACTERIZATION OF PRINCIPAL SOLUBLE-ANTIGENS ASSOCIATED WITH CORONAVIRUS OF TRANSMISSIBLE GASTROENTERITIS BY COMPLEMENT-FIXATION AND IMMUNODIFFUSION
复制标题
DOI：
10.1128/iai.13.2.521-526.1976
复制
发表时间：
1976-01-01
影响因子：
3.1
通讯作者：
JENSEN, MT
中科院分区：
文献类型：
--
作者：
STONE, SS;KEMENY, LJ;JENSEN, MT
以猪传染性胃肠炎（TGE）高免血清为抗体源，建立了检测TGE病毒抗原的微量补体结合试验。TGE恢复期猪血清不能固定补体。接种猪睾丸细胞后36-48 h获得最大病毒和可溶性抗原（SA）滴度。细胞相关病毒和SA滴度高于培养液中的病毒和SA滴度，培养液必须浓缩20倍。在琼脂免疫扩散试验（ID）中用作抗原之前。通过蔗糖密度梯度离心，SA具有1.10 g/ml的浮力密度，并且可以与在1.19 g/ml区域中条带化的病毒分离。3个TGE分离株的病毒和SA具有相同的浮力密度。加热和蛋白水解酶消化确立了SA的蛋白质性质。通过CF和ID测定，SA粗品和纯化制剂之间存在稳定性差异。在兔中制备的抗SA的抗体中和了TGE病毒。
A microtiter complement fixation (CF) test to detect transmissible gastroenteritis (TGE) viral antigen was developed, using TGE hyperimmune pig serum as an antibody source. Sera from TGE convalescent pigs did not fix complement by this test. Maximal virus and soluble antigen (SA) titers were obtained 36-48 h after inoculation of swine testes cells. Cell-associated virus and SA titers were higher than those in the culture fluid, which had to be concentrated 20.times. before use as antigen in agar immunodiffusion tests (ID). By sucrose density-gradient centrifugation, the SA had a buoyant density of 1.10 g/ml and could be separated from the virus that banded in the 1.19-g/ml region. Virus and SA from 3 different isolates of TGE had the same buoyant densities. Heating and proteolytic enzyme digestion established the protein nature of the SA. As assayed by CF and ID, there were stability differences between crude and purified preparations of SA. Antibody prepared in rabbits against the SA neutralized the TGE virus.