A comparison of human and porcine acellularized dermis: Interactions with human fibroblasts in vitro
A comparison of human and porcine acellularized dermis: Interactions with human fibroblasts in vitro
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DOI：
10.1097/01.prs.0000204567.28952.9d
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发表时间：
2006-03-01
影响因子：
3.6
通讯作者：
Semple, JL
中科院分区：
文献类型：
--
作者：
Armour, AD;Fish, JS;Semple, JL
背景：从异种移植材料中获得的真皮替代品需要复杂的加工，而且成本相当高。脱细胞猪真皮是一种容易获得的材料，具有最小的免疫原性。本研究的目的是评估脱细胞化的猪真皮作为人成纤维细胞的支架。方法：采用体外方法观察成纤维细胞在猪脱细胞真皮基质上的粘附、增殖和迁移。以脱细胞人真皮层为对照。结果：猪脱细胞真皮基质作为人成纤维细胞支架的效果不如人脱细胞真皮基质。人脱细胞真皮基质样品（83%,n = 24; p < 0.05）比猪脱细胞真皮基质样品（31%,n = 49）在细胞种子表面下显示成纤维细胞浸润。人脱细胞真皮基质表面以下的成纤维细胞浸润量（平均每切片1072 +/- 80个细胞，n = 16个样本）显著高于猪脱细胞真皮基质（平均每切片301 +/- 48个细胞，n = 16个样本）。与人脱细胞真皮基质相比，成纤维细胞在离猪脱细胞真皮基质表面的迁移距离显著减少（p < 0.05）（分别为78.8%和38.3%的细胞在离表面150 μ m内迁移；n = 5）。成纤维细胞在猪脱细胞真皮基质（n = 9）上比在人脱细胞真皮基质上增殖更快（p < 0.05）（细胞数量分别增加7.4倍和1.8倍；人脱细胞真皮基质的细胞数量分别增加71 = 9）。两种材料在成纤维细胞粘附方面没有差异（猪和人脱细胞真皮基质每切片平均粘附细胞分别为8120和7436；每组n = 20; p > 0.05）。结论：初步研究结果表明，人成纤维细胞在猪和人脱细胞真皮细胞-基质相互作用中的行为可能存在实质性差异。
Background: Dermal substitutes derived from xenograft materials require elaborate processing at a considerable cost. Acellularized porcine dermis is a readily available material associated with minimal immunogenicity. The objective of this study was to evaluate acellularized pig dermis as a scaffold for human fibroblasts.Methods: In vitro methods were used to evaluate fibroblast adherence, proliferation, and migration on pig acellularized dermal matrix. Acellular human dermis was used as a control.Results: Pig acellularized dermal matrix was found to be inferior to human acellularized dermal matrix as a scaffold for human fibroblasts. Significantly more samples of human acellularized dermal matrix (83 percent, n = 24; p < 0.05) demonstrated fibroblast infiltration below the cell-seeded surface than pig acellularized dermal matrix (31 percent, n. = 49). Significantly more (p < 0.05) fibroblasts infiltrated below the surface of human acellularized dermal matrix (mean, 1072 +/- 80 cells per section; n = 16 samples) than pig acellularized dermal matrix (mean, 301 +/- 48 cells per section; n = 16 samples). Fibroblasts migrated significantly less (p < 0.05) distance from the cell-seeded pig acellularized dermal matrix surface than in the human acellularized dermal matrix (78.8 percent versus 38.3 percent cells within 150 mu m from the surface, respectively; n = 5). Fibroblasts proliferated more rapidly (p < 0.05) on pig acellularized dermal matrix (n = 9) than on the human acellularized dermal matrix (7.4-fold increase in cell number versus 1.8-fold increase, respectively; 71 = 9 for human acellularized dermal matrix). There was no difference between the two materials with respect to fibroblast adherence (8120 versus 7436 average adherent cells per section, for pig and human acellularized dermal matrix, respectively; n. = 20 in each group; p > 0.05).Conclusion: Preliminary findings Suggest that substantial differences may exist between human fibroblast behavior in cell-matrix interactions of porcine and human acellularized dermis.