Expression of the transformation-sensitive protein "cyclin" in normal human epidermal basal cells and simian virus 40-transformed keratinocytes.
Expression of the transformation-sensitive protein "cyclin" in normal human epidermal basal cells and simian virus 40-transformed keratinocytes.
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转化敏感蛋白“细胞周期蛋白”在正常人表皮基底细胞和猿猴病毒40转化的角质形成细胞中的表达。
DOI：
10.1073/pnas.81.10.3128
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发表时间：
1984
影响因子：
11.1
通讯作者：
A. Celis
中科院分区：
文献类型：
--
作者：
J. Celis;S. Fey;P. Larsen;A. Celis
在人表皮基底细胞中获得了一个高度富集的细胞群，并使用针对表皮中各种细胞类型的特异性抗体对其进行了表征。对基底细胞和猴病毒40转化角质形成细胞中[35S]蛋氨酸标记多肽的定量二维凝胶电泳（等电聚焦）分析显示，与转化细胞相比，基底细胞合成的核转化敏感蛋白cyclin的量非常低（不到总蛋白的0.02%），而转化细胞则组成性地合成该蛋白（占总蛋白的0.15%）。在整个人类表皮中观察到非常低水平的细胞周期蛋白，对两个基底瘤的初步研究表明，这些肿瘤中有显著的细胞周期蛋白合成。至少在培养细胞的情况下，使用免疫沉淀周期蛋白的增殖细胞核抗原抗体进行的免疫荧光研究证实了上述观察结果。综上所述，这些结果支持细胞周期蛋白可能是控制细胞增殖途径的核心组成部分的观点。
A cell population highly enriched in human epidermal basal cells has been obtained and characterized by using antibodies specific for various cell types in the epidermis. Quantitative two-dimensional gel electrophoretic analysis (isoelectric focusing) of [35S]methionine-labeled polypeptides from basal cells and simian virus 40-transformed keratinocytes showed that the basal cells synthesize very low amounts (less than 0.02% of the total protein) of the nuclear, transformation-sensitive protein cyclin as compared to the transformed cells, which synthesize this protein constitutively (0.15% of the total protein). Very low levels of cyclin were observed in total human epidermis, and preliminary studies of two basaliomas have shown a significant synthesis of this protein in these tumors. Immunofluorescence studies using antibodies to proliferating cell nuclear antigen that immunoprecipitate cyclin confirmed the above observations at least in the case of the cultured cells. Taken together, these results support the notion that cyclin may be a central component of the pathway(s) that controls cell proliferation.