Lokalisierung des Monogalaktosyldiglycerids in Thylakoidmembranen mit serologischen Methoden / Serological Localisation of Monogalactosyl Diglyceride in Thylakoid Membranes
Lokalisierung des Monogalaktosyldiglycerids in Thylakoidmembranen mit serologischen Methoden / Serological Localisation of Monogalactosyl Diglyceride in Thylakoid Membranes
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Lokalisierung des Monogalactosyldiglycerids in Thylakoidmembranen mit serologischen Methoden / 类囊体膜中单半乳糖二甘油酯的血清学定位
DOI：
10.1515/znb-1972-0719
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
A. Radunz
中科院分区：
文献类型：
--
作者：
A. Radunz
在不同叶绿体制剂的抗血清中发现了针对单半乳糖二甘油酯的免疫球蛋白。通过注射吸附在甲基化的牛血清白蛋白上的单半乳糖二甘油酯，获得了一种特异性的抗脂质血清。对单糖和寡糖以及单半乳糖和双半乳糖甘油的抑制试验表明，免疫球蛋白被定向到β-半乳糖-(1→1)-甘油构型。双半乳糖甘油三酯和亚喹诺醇甘油三酯与抗血清无反应。免疫球蛋白为Ig m型。抗单半乳糖二甘油酯的血清能使无基质的叶绿体凝集。通过超声和分馏离心得到的类囊体膜碎片、类囊体和类囊体堆都有沉淀。在含有基质的叶绿体制备过程中，在叶绿体膜破裂和基质丢失后发生凝集。超声处理的片层系统比无基质的叶绿体结合更大量的单半乳糖双甘油酯免疫球蛋白。从这些实验中可以得出结论，一些叶绿体的单半乳糖二甘油酯位于类囊体的表面。
Immunoglobulins to monogalactosyl diglyceride have been found in antisera to different chloroplast preparations. A specific antiserum to this lipid has been obtained by injection of monogalactosyl diglyceride which was adsorbed onto methylated bovine serum albumin. Inhibition tests with mono- and oligosaccharides, as well as with mono- and digalactosyl glycerol, showed that the immunoglobulin was directed to the β-galactosyl-(1 → 1) -glycerol configuration. Digalactosyl diglyceride and sulphoquinovosyl diglyceride did not react with the antiserum. The immunoglobulin is of the Ig M-type. The antiserum to monogalactosyl diglyceride agglutinates stroma-freed chloroplasts. Fragments of the thylakoid membrane, obtained by ultrasonication and fractioning centrifugation, thylakoids and thylakoid stacks are all precipitated. In a preparation of stroma-containing chloroplasts agglutination takes place but after rupture of the chloroplast membrane and loss of the stroma. The ultrasonicated lamellar system binds a larger amount of the monogalactosyl diglyceride immunoglobulins than does stroma-freed chloroplasts. It is concluded from these experiments that some of the chloroplasts monogalactosyl diglyceride is located at the surface of the thylakoids.