Quantitative measurements of chromatin modification dynamics during zygotic genome activation
Quantitative measurements of chromatin modification dynamics during zygotic genome activation
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DOI：
10.1101/601393
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发表时间：
2019-04
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通讯作者：
Y. Sato;Lennart Hilbert;H. Oda;Yinan Wan;John M. Heddleston;T. Chew;V. Zaburdaev;Philipp J. Keller;T. Lionnet;N. Vastenhouw;H. Kimura
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文献类型：
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作者：
Y. Sato;Lennart Hilbert;H. Oda;Yinan Wan;John M. Heddleston;T. Chew;V. Zaburdaev;Philipp J. Keller;T. Lionnet;N. Vastenhouw;H. Kimura
组蛋白翻译后修饰（PTM）是关键的基因表达调控因子，但其在发育过程中的快速动态仍然难以捕捉。我们描述了一种方法，图像荧光抗体片段定量PTM富集和活性转录在定义的位点在活斑马鱼胚胎合子基因组激活。该技术揭示了组蛋白H3 Lys27乙酰化（H3K27ac）在基因组激活之前在特定位点和全局上的急剧增加。
Histone posttranslational modifications (PTMs) are key gene expression regulators, but their rapid dynamics during development remain difficult to capture. We describe an approach that images fluorescent antibody fragments to quantify PTM enrichment and active transcription at defined loci during zygotic genome activation in living zebrafish embryos. The technique reveals a drastic increase in histone H3 Lys27 acetylation (H3K27ac) before genome activation both at a specific locus and globally.