Cloning, characterization, and biological function analysis of the SidT2 gene from Siniperca chuatsi
Cloning, characterization, and biological function analysis of the SidT2 gene from Siniperca chuatsi
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鳜鱼SidT2基因的克隆、鉴定及生物学功能分析
DOI：
10.1016/j.dci.2011.02.003
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发表时间：
2011-06-01
影响因子：
2.9
通讯作者：
He, Jianguo
中科院分区：
文献类型：
--
作者：
Ren, Ruiwen;Xu, Xiaopeng;He, Jianguo
系统性RNA干扰缺陷蛋白(SID)-1在细胞dsRNA摄取中起重要作用。我们从鳜鱼（sininiperca chuatsi）中鉴定出ScSidT2基因，这是鱼类中首次研究的SID-1同源物。ScSidT2 mRNA长3380 bp，包含一个2568 bp的开放阅读框，编码一个855个氨基酸的蛋白，包含一个n端信号肽和十个假定的跨膜结构域。分析了ScSidT2在健康鱼体内的组织分布和感染脾肾坏死病毒（ISKNV）的鱼体内的表达谱。过表达ScSidT2蛋白可以显著增加外源dsRNA在黑头鱼（FHM）上皮细胞中的摄取。在虎蛙病毒（TFV）感染的FHM细胞中，过表达ScSidT2可以抑制病毒的产生，并且通过添加病毒特异性dsRNA可以显著增强这一机制。在鳜鱼鱼苗细胞中，ScSidT2蛋白被特异性抗血清中和后可促进ISKNV感染和繁殖。这些结果表明ScSidT2可能与宿主的抗病毒防御机制有关。(C) 2011 Elsevier Ltd.版权所有。
The systemic RNA interference defective protein (SID)-1 plays an important role in dsRNA uptake in cells. We identified the ScSidT2 gene from the mandarin fish (Siniperca chuatsi), which is the first-studied SID-1 homolog in fish. ScSidT2 mRNA is 3380 bp long and contains a 2568-bp open reading frame that encodes an 855-amino-acid protein with an N-terminal signal peptide and ten putative transmembrane domains. Tissue distribution profile in healthy fish and expression profiles of ScSidT2 in infectious spleen and kidney necrosis virus (ISKNV)-infected fish were analyzed. Overexpression of the ScSidT2 protein in fathead minnow (FHM) epithelial cells could remarkably increase the uptake of exogenous dsRNA. In tiger frog virus (TFV)-infected FHM cells, overexpression of ScSidT2 could suppress virus production, and this mechanism could be significantly enhanced by adding virus-specific dsRNA. In mandarin fish fry cells, ISKNV infection and reproduction could be promoted when the ScSidT2 protein was neutralized with specific antisera. These results suggest that ScSidT2 could be associated with the host's antiviral defense mechanism. (C) 2011 Elsevier Ltd. All rights reserved.