A low-density lipoprotein receptor (LDLR) class A domain-containing C-type lectin from Litopenaeus vannamei plays opposite roles in antibacterial and antiviral responses
A low-density lipoprotein receptor (LDLR) class A domain-containing C-type lectin from Litopenaeus vannamei plays opposite roles in antibacterial and antiviral responses
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来自南美白对虾的低密度脂蛋白受体 (LDLR) A 类结构域 C 型凝集素在抗菌和抗病毒反应中发挥相反的作用。
DOI：
10.1016/j.dci.2018.11.002
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发表时间：
2019-03-01
影响因子：
2.9
通讯作者：
Xu, Xiaopeng
中科院分区：
文献类型：
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作者：
Liang, Zhiwei;Yang, Linwei;Xu, Xiaopeng
C型凝集素（C-type lectin，CTL）是一类含有糖识别结构域的模式识别受体（pattern recognition receptors，PRRs），在天然免疫中起重要作用。在许多甲壳类动物中已经鉴定出含有额外的低密度脂蛋白受体（LDLR）A类结构域（LdlrCTL）的CTL，但其在免疫应答中的功能大多是未知的。本研究从太平洋白色凡纳滨对虾（Litopenaeus vannamei，LvLdlrCTL）中分离到一种新的LdlrCTL，与已报道的甲壳类LdlrCTL具有高度同源性。LvLdlrCTL在血细胞中高表达，免疫刺激后表达上调。沉默LdlrCTL可显著促进副溶血性弧菌对对虾的感染，但抑制白色斑点综合征病毒（WSSV）对对虾的感染，表明LdlrCTL在抗菌和抗病毒反应中可能起相反的作用。LdlrCTL具有抗细菌和真菌的凝集活性，并能增强血细胞的吞噬作用。此外，LdlrCTL沉默后，许多免疫效应基因和信号通路组分的表达发生了显著变化，表明LdlrCTL可能参与了免疫调节。
C-type lectins (CTLs) are a group of pattern recognition receptors (PRRs) that contain carbohydrate recognition domains and play important roles in innate immunity. CTLs that contain an additional low-density lipoprotein receptor (LDLR) class A domain (LdlrCTL) have been identified in many crustaceans, but their functions in immune responses are mostly unknown. In this study, a novel LdlrCTL was identified from pacific white shrimp Litopenaeus vannamei (LvLdlrCTL), which showed high homology with previously reported crustacean LdlrCTLs. LvLdlrCTL was highly expressed in hemocytes and its expression was up-regulated after immune stimulations. Silencing of LdlrCTL significantly promoted infection of shrimp by Vibrio parahaemolyticus but inhibited infection by white spot syndrome virus (WSSV), suggesting that LdlrCTL could play opposite roles in antibacterial and antiviral responses. LdlrCTL exhibited agglutination activity against bacteria and fungi and could potentiate the phagocytosis of hemocytes. Moreover, the expression of many immune effector genes and signalling pathway components was significantly changed in LdlrCTL-silenced shrimp, indicating that LdlrCTL could be involved in immune regulation.