On the origin of family 1 plant glycosyltransferases
On the origin of family 1 plant glycosyltransferases
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DOI：
10.1016/s0031-9422(02)00558-7
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发表时间：
2003-02-01
期刊：
影响因子：
3.8
通讯作者：
Bak, S
中科院分区：
文献类型：
--
作者：
Paquette, S;Moller, BL;Bak, S
高度分化的多基因家族的系统发育通常难以验证，但可以通过包含系统发育之外的数据来证实，例如特征基序、内含子剪接位点保守性、保守残基的独特替换、相似的基因功能和外群。家族I糖基转移酶（UGTs）包括这样一个高度分化的多基因家族。植物、动物、真菌、细菌和病毒的ugt的系统发育比较表明，植物ugt代表三个不同的分支。大多数植物序列似乎是单系的，并在动物/真菌/植物界分叉后分化。这两个小分支含有甾醇和脂质糖基转移酶，并且与非植物序列具有更多的同源性。脂质糖基转移酶分支与细菌脂质糖基转移酶同源，反映了叶绿体的细菌起源。完整测序的拟南芥基因组包含120个ugt，其中包括8个明显的假基因。通过对拟南芥UGT多基因家族的完整系统发育分析，包括内含子-外显子组织和染色体定位，证实了植物糖基转移酶的系统发育。2003爱思唯尔科学有限公司版权所有。
The phylogeny of highly divergent multigene families is often difficult to validate but can be substantiated by inclusion of data outside of the phylogeny, such as signature motifs, intron splice site conservation, unique substitutions of conserved residues, similar gene functions, and out groups. The Family I Glycosyltransferases (UGTs) comprises such a highly divergent, polyphyletic multigene family. Phylogenetic comparisons of UGTs from plants, animals, fungi, bacteria, and viruses reveal that plant UGTs represent three distinct clades. The majority of the plant sequences appears to be monophyletic and have diverged after the bifurcation of the animal/fungi/plant kingdoms. The two minor clades contain the sterol and lipid glycosyltransferases and each show more homology to non-plant sequences. The lipid glycosyltransferase clade is homologous to bacterial lipid glycosyltransferases and reflects the bacterial origin of chloroplasts. The fully sequenced Arabidopsis thaliana genome contains 120 UGTs including 8 apparent pseudogenes. The phylogeny of plant glycosyltransferases is substantiated with complete phylogenetic analysis of the A. thaliana UGT multigene family, including intron-exon organization and chromosomal localization. (C) 2003 Elsevier Science Ltd. All rights reserved.