Chemoautotrophic production of gaseous hydrocarbons, bioplastics and osmolytes by a novel Halomonas species.
Chemoautotrophic production of gaseous hydrocarbons, bioplastics and osmolytes by a novel Halomonas species.
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DOI：
10.1186/s13068-023-02404-1
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发表时间：
2023-10-11
期刊：
影响因子：
--
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中科院分区：
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通过微生物物种生产价值相对较低的大宗商品化学品和燃料需要逐步改变方法，以降低与规模发酵相关的资本和运营成本。利用盐单胞菌属的健壮且嗜盐的工业宿主生物体可以显著降低生物制造成本，因为它们能够在非无菌条件下使用废弃的生物源原料在海水中生长。我们描述了一种新的兼性化能自养的盐单胞菌从天然盐泉中分离的罗旺盐单胞菌。我们调查了该物种的能力，以产生四氢嘧啶，化合物的相当大的工业利益，在异养条件下。用H.在以硫代硫酸盐作为能量源的矿物培养基中证实了rowanensis。基因组测序表明碳固定通过还原性三羧酸循环进行，而不是Calvin-Bensen-Bassham循环。支持化能自养的能量产生机制是未知的，由于没有注释的SOX基硫代硫酸盐介导的能量转换系统。我们进一步研究了分离的H.通过展示在异养和自养CO2固定生长条件下气态烃（生物丙烷）、生物塑料（聚-3-羟基丁酸酯）和渗透调节剂（四氢嘧啶）的产生，对rowanensis进行了研究。这项概念验证研究说明了招募环境隔离物作为化学品生物制造工业宿主的价值，其中CO2利用可以取代或增加非无菌工业化生物反应器中生物原料的使用。在线版本包含补充材料，可通过10.1186/s13068-023-02404-1获得。新的嗜盐分离物罗旺盐单胞菌可用于在中性pH下的化学品和燃料生产。rowanensis可能是一种兼性化能无机营养生物，能够在硫代硫酸盐存在下固定CO2。重组H.罗旺人H.在化学品和先进合成燃料的非无菌生产中，在线版本包含补充材料，可通过10.1186/s13068-023-02404-1获得。
Production of relatively low value, bulk commodity chemicals and fuels by microbial species requires a step-change in approach to decrease the capital and operational costs associated with scaled fermentation. The utilisation of the robust and halophilic industrial host organisms of the genus Halomonas could dramatically decrease biomanufacturing costs owing to their ability to grow in seawater, using waste biogenic feedstocks, under non-sterile conditions. We describe the isolation of Halomonas rowanensis, a novel facultative chemoautotrophic species of Halomonas from a natural brine spring. We investigated the ability of this species to produce ectoine, a compound of considerable industrial interest, under heterotrophic conditions. Fixation of radiolabelled NaH14CO3 by H. rowanensis was confirmed in mineral medium supplied with thiosulfate as an energy source. Genome sequencing suggested carbon fixation proceeds via a reductive tricarboxylic acid cycle, and not the Calvin–Bensen–Bassham cycle. The mechanism of energy generation to support chemoautotrophy is unknown owing to the absence of an annotated SOX-based thiosulfate-mediated energy conversion system. We investigated further the biotechnological potential of the isolated H. rowanensis by demonstrating production of the gaseous hydrocarbon (bio-propane), bioplastics (poly-3-hydroxybutyrate) and osmolytes (ectoine) under heterotrophic and autotrophic CO2 fixation growth conditions. This proof-of-concept study illustrates the value of recruiting environmental isolates as industrial hosts for chemicals biomanufacturing, where CO2 utilisation could replace, or augment, the use of biogenic feedstocks in non-sterile, industrialised bioreactors. The online version contains supplementary material available at 10.1186/s13068-023-02404-1. New halophilic isolate Halomonas rowanensis is useful for chemicals and fuel production at neutral pH. H. rowanensis is a likely facultative chemolithotroph capable of CO2 fixation in the presence of thiosulfate. Propane production occurred under both heterotrophic and autotrophic growth conditions with recombinant H. rowanensis. Potential application of H. rowanensis in the non-sterile production of chemicals and advanced synthetic fuels. The online version contains supplementary material available at 10.1186/s13068-023-02404-1.
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微生物生物烷天然气生产的启动子工程。
DOI：
10.1093/synbio/ysaa022
发表时间：
2020
期刊：
Synthetic biology (Oxford, England)
影响因子：
--
作者：
Trisrivirat D;Hughes JMX;Hoeven R;Faulkner M;Toogood H;Chaiyen P;Scrutton NS
通讯作者：
Scrutton NS
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
4.8
作者：
Dou, Zhicheng;Heinhorst, Sabine;Cannon, Gordon C.
通讯作者：
Cannon, Gordon C.
影响因子：
6.7
作者：
Faulkner M;Rodriguez-Ramos J;Dykes GF;Owen SV;Casella S;Simpson DM;Beynon RJ;Liu LN
通讯作者：
Liu LN
影响因子：
--
作者：
Leung, Ivanhoe K. H.;Krojer, Tobias J.;Schofield, Christopher J.
通讯作者：
Schofield, Christopher J.