An LC-QToF MS based method for untargeted metabolomics of human fecal samples
An LC-QToF MS based method for untargeted metabolomics of human fecal samples
复制标题
DOI：
10.1007/s11306-020-01669-z
复制
发表时间：
2020-04-03
期刊：
影响因子：
3.6
通讯作者：
Landberg, Rikard
中科院分区：
文献类型：
--
作者：
Cheng, Ken;Brunius, Carl;Landberg, Rikard
在非靶向人类粪便代谢组学的样品制备方面缺乏共识。目的为高通量LC-MS提供代谢物覆盖面广的样品制备方法。方法以提取溶剂、溶剂比和新鲜冻干样品的代谢物特征质量为指标进行评价。结果每克湿粪含5毫升甲醇时，新鲜、冷冻和冻干样品的代谢产物覆盖面广，信号强度和饱和度达到最佳平衡。冷冻干燥不影响SCFA，推荐使用，因为便于将其归一化为干物质。结论所建立的样品制备方法简单、高效，适用于大规模的人类粪便代谢组学研究。
Introduction Consensus in sample preparation for untargeted human fecal metabolomics is lacking. Objectives To obtain sample preparation with broad metabolite coverage for high-throughput LC-MS. Methods Extraction solvent, solvent ratio and fresh frozen-vs-lyophilized samples were evaluated by metabolite feature quality. Results Methanol at 5 mL per g wet feces provided a wide metabolite coverage with optimal balance between signal intensity and saturation for both fresh frozen and lyophilized samples. Lyophilization did not affect SCFA and is recommended because of convenience in normalizing to dry matter. Conclusion The suggested sample preparation is simple, efficient and suitable for large-scale human fecal metabolomics.