Detection of pyroptosis by measuring released lactate dehydrogenase activity.
Detection of pyroptosis by measuring released lactate dehydrogenase activity.
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DOI：
10.1007/978-1-62703-523-1_7
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Miao, Edward A
中科院分区：
文献类型：
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作者：
Rayamajhi, Manira;Zhang, Yue;Miao, Edward A
焦亡是一种依赖于半胱氨酸蛋白酶Caspase-1激活的程序性炎性细胞死亡形式。在本章中，我们描述了一种酶法检测乳酸脱氢酶（LDH）释放的死亡或垂死的细胞在焦亡使用市售试剂盒。我们还讨论了另一种简单和成本效益的方法来衡量LDH改编自德克尔等。
Pyroptosis is a form of programmed, inflammatory cell death that is dependent on the activation of a cysteine protease Caspase-1. In this chapter, we describe an enzymatic assay for the detection of lactate dehydrogenase (LDH) released by dead or dying cells during pyroptosis using a commercially available kit. We also discuss another simple and cost-effective method to measure LDH adapted from Decker et al..