A family of brevinin-2 peptides with potent activity against Pseudomonas aeruginosa from the skin of the Hokkaido frog, Rana pirica
A family of brevinin-2 peptides with potent activity against Pseudomonas aeruginosa from the skin of the Hokkaido frog, Rana pirica
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DOI：
10.1016/j.regpep.2003.12.003
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发表时间：
2004-05-15
影响因子：
--
通讯作者：
Pál, T
中科院分区：
文献类型：
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作者：
Conlon, JM;Sonnevend, A;Pál, T
从北海道蛙（Rana pirica）的皮肤中提取了9种具有不同程度抗菌活性的肽。五个结构相关的肽被鉴定为brevinin-2家族的成员。这些肽对革兰氏阴性菌（大肠杆菌、铜绿假单胞菌、阴沟肠杆菌、肺炎克雷伯菌）和革兰氏阳性菌（金黄色葡萄球菌）的参比菌株有活性，但溶血活性相对较低。最丰富的肽brevinin-2PRa （680 nmol/g干燥皮肤重量）对一系列铜绿假单胞菌的临床分离株表现出高效[最小抑制浓度（MIC）值在6至12 μ m之间]。此外，当NaCl浓度达到200mM时，活性不受影响。基于brevinin-2多肽初级结构的进化分析支持了日本山褐蛙（Rana ornativentris）与日本山褐蛙的亲缘关系。从提取物中还分离出了ranatuerin-2家族的一个肽和brevinin-1家族的一个强溶血肽，以及temporin家族的两个成员，即temporin- 1pra （ILPILGNLLNGLL.NH2）和temporin- 1prb (ILPILGNLLNSLL.NH2)，它们典型地缺乏碱性氨基酸残基，仅表现出非常弱的抗菌和溶血活性。(C) 2004 Elsevier B.V.版权所有
Nine peptides displaying varying degrees of antimicrobial activity were extracted from the skin of the Hokkaido frog, Rana pirica. Five structurally related peptides were identified as members of the brevinin-2 family. These peptides were active against reference strains of Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, Klebsiella pneumoniae) and Gram-positive (Staphlococcus aureus) bacteria but displayed relatively low hemolytic activity. The most abundant peptide, brevinin-2PRa (680 nmol/g weight of dry skin) showed high potency [minimal inhibitory concentration (MIC) values between 6 and 12 muM] against a range of clinical isolates of P aeruginosa. In addition, activity was unaffected by NaCl concentrations up to 200mM. Cladistic analysis based on the primary structures of brevinin-2 peptides supports a close phylogenetic relationship between R. pirica and Japanese mountain brown frog Rana ornativentris. One peptide of the ranatuerin-2 family and one strongly hemolytic peptide of the brevinin-1 family were also isolated from the extract along with two members of the temporin family, temporin-1PRa (ILPILGNLLNGLL.NH2) and temporin-1PRb (ILPILGNLLNSLL.NH2) that atypically lacked basic amino acid residues and showed only very weak antimicrobial and hemolytic activity. (C) 2004 Elsevier B.V. All rights reserved.