Evaluation of Antiherpetic Compounds Using a Gastric Cancer Cell Line: Pronounced Activity of BVDU against Herpes Simplex Virus Replication
Evaluation of Antiherpetic Compounds Using a Gastric Cancer Cell Line: Pronounced Activity of BVDU against Herpes Simplex Virus Replication
复制标题
使用胃癌细胞系评价抗疱疹化合物：BVDU 对抗单纯疱疹病毒复制的显着活性
DOI：
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发表时间：
1996
影响因子：
2.6
通讯作者：
S. Shigeta
中科院分区：
文献类型：
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作者：
E. Kodama;A. Igarashi;S. Mori;K. Hashimoto;Toshimitsu Suzuki;E. De Clercq;S. Shigeta
我们建立了一种快速、简便的体外筛选抗单纯疱疹病毒（HSV）抗病毒药物的方法。该方法基于抑制胃腺癌MKN-28细胞中HSV诱导的致细胞病变性，如通过MTT比色试验监测的。在评价其抗HSV-1和HSV-2活性的各种化合物中，溴夫定（BVDV）是最有效的。当将MKN-28细胞中抗疱疹药物的50%有效浓度（EC 50）值与人胚肺MRC-5细胞中的值进行比较时，除BVDV外，所有化合物在MKN-28细胞中均显示出较高的EC 50值。对于BVDV，MKN-28细胞中的EC 50值是MRC-5细胞中EC 50值的0.8（HSV-1）和0.036（HSV-2）倍。因此，MKN-28细胞中的BVDV抗HSV-2活性是MRC-5细胞中的27.5倍。MKN-28胃癌细胞可用于快速筛选抗HSV药物，特别是可用于治疗胃肠道疱疹感染中的胃肠道HSV感染的药物。
We developed a rapid and simple method for the screening of antiviral agents against herpes simplex virus (HSV) in a model of gastrointestinal herpetic infection in vitro. This method was based on inhibition of HSV‐induced cytopathogenicity in gastric adenocarcinoma MKN‐28 cells, as monitored by an MTT colorimetric assay. From the various compounds that were evaluated for their activity against HSV‐1 and HSV‐2, brivudine (BVDU) emerged as the most effective. When the 50% effective concentration (EC50) values of the antiherpes agents in MKN‐28 cells were compared with those in human embryo lung MRC‐5 cells, all compounds, except for BVDU, showed higher EC50 values in MKN‐28 cells. For BVDU the EC50 values in MKN‐28 cells were 0.8 (HSV‐1) and 0.036 (HSV‐2) times the EC50 values in MRC‐5 cells. Thus BVDU was 27.5 times more active against HSV‐2 in MKN‐28 cells than in MRC‐5 cells. The MKN‐28 gastric cancer cells may be useful for the rapid screening of anti‐HSV agents and, in particular, those that may be useful in therapy of gastrointestinal HSV infections in gastrointestinal herpetic infection.
DOI：
10.1093/infdis/148.6.1077
发表时间：
1983-12
期刊：
The Journal of infectious diseases
影响因子：
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作者：
J. Wade;C. Mclaren;J. Meyers
通讯作者：
J. Wade;C. Mclaren;J. Meyers