THE CHROMOSOME 14 BREAKPOINT IN NEOPLASTIC B-CELLS WITH THE T(11-14) TRANSLOCATION INVOLVES THE IMMUNOGLOBULIN HEAVY-CHAIN LOCUS
THE CHROMOSOME 14 BREAKPOINT IN NEOPLASTIC B-CELLS WITH THE T(11-14) TRANSLOCATION INVOLVES THE IMMUNOGLOBULIN HEAVY-CHAIN LOCUS
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DOI：
10.1073/pnas.81.13.4144
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
CROCE, CM
中科院分区：
文献类型：
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作者：
ERIKSON, J;FINAN, J;CROCE, CM
肿瘤细胞取自一例B细胞型慢性淋巴细胞白血病患者，其11号染色体(Q13)和14号染色体(Q32)与小鼠浆细胞瘤细胞进行了染色体互换。对杂合细胞进行了人免疫球蛋白MU的存在、重排和表达的研究。链式轨迹。表达的.MU。Chain基因明显位于正常14号染色体上，而14Q+易位染色体携带排除的Ig常数(C)区。链等位基因(C.mu.)但不包含可变(V)区H链基因(VH)。被排除者的H链连接区DNA(JH)。Chain基因位于14Q+染色体上；白血病细胞的染色体断裂发生在JH区内或5‘端的染色体区域。从逻辑上讲，一个名为bcl1的基因位于11号染色体的Q13带上，通过易位到14q+上重排的H链座位附近而被激活，有助于t(11；14)染色体易位的B细胞的肿瘤性转化。
Neoplastic cells were hybridized from a patient with chronic lymphocytic leukemia of the B-cell type, which carried a reciprocal chromosomal translation between chromosomes 11 (q13) and 14 (q32) with mouse plasmacytoma cells. The hybrid cells were studied for the presence, rearrangement and expression of the human Ig .mu. chain locus. The expressed .mu. chain gene is apparently located on the normal chromosome 14, whereas the 14q+ translocation chromosome carries the excluded Ig constant (C) region .mu. chain allele (C.mu.) but does not contain variable (V) region H chain genes (VH). The H chain joining region DNA (JH) of the excluded .mu. chain gene is on the 14q+ chromosome; the chromosomal break observed in the leukemic cells occurred in a chromosomal region within or 5'' of the JH region. Logically a gene, with the suggested name bcl-1, is located on band q13 of chromosome 11 and is activated by its translocation into close proximity with the rearranged H chain locus on chromosome 14q+, contributing to the neoplastic transformation of the B cells with the t(11;14) chromosomal translocation.