TRANSFORMATION OF MYOSIN IN CROSS-INNERVATED RAT MUSCLES
TRANSFORMATION OF MYOSIN IN CROSS-INNERVATED RAT MUSCLES
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DOI：
10.1113/jphysiol.1971.sp009393
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发表时间：
1971-01-01
影响因子：
5.5
通讯作者：
CLOSE, RI
中科院分区：
文献类型：
--
作者：
BARANY, M;CLOSE, RI
1. 测定了大鼠正常（N - EDL, N - SOL）、自神经支配（S - EDL, S - SOL）和交叉神经支配（X - EDL, X - SOL）指长伸肌（EDL）和比目鱼肌（SOL）在35℃下的等距抽搐和强直收缩的特征。这些肌肉随后被用于肌球蛋白和肌动球蛋白特性的生化分析。X - EDL的肌凝蛋白和肌动球蛋白的atp酶活性下降到N - SOL或S - SOL的水平，X - SOL的atp酶活性接近N - EDL或S - EDL的水平。在各种atp酶活性中，肌凝蛋白的肌动蛋白和Mg2+激活的atp酶活性以及肌动球蛋白的Mg2+激活的atp酶活性与肌肉的内在缩短速度的相关性最高。正常肌肉、自神经支配肌肉和交叉神经支配肌肉的肌球蛋白与肌动蛋白的超沉淀速率与肌肉收缩速度成正比。pH曲线和ATP诱导的二硝基苯基化反应表明，X - EDL肌球蛋白的结构被改变为N - EDL或S - SOL的结构，X - SOL肌球蛋白的结构被改变为N - EDL或S - EDL的结构。正常、自神经支配和交叉神经支配的指长伸肌和比目鱼肌肌球蛋白的产生没有差异。
1. The characteristics of isometric twitch and tetanic contractions have been determined for normal (N‐EDL, N‐SOL), self‐innervated (S‐EDL, S‐SOL) and cross‐innervated (X‐EDL, X‐SOL) extensor digitorum longus (EDL) and soleus (SOL) muscles of the rat at 35° C. The muscles were then used for biochemical analyses of properties of myosin and actomyosin.2. The ATPase activities of myosin and actomyosin of X‐EDL decreased to the level of those of N‐SOL or S‐SOL, and the ATPase activities of X‐SOL approached those of N‐EDL or S‐EDL. Of the various ATPase activities, the actin‐ and Mg2+‐activated ATPase activity of myosin and the Mg2+‐activated ATPase activity of actomyosin showed the highest degree of correlation with the intrinsic speed of shortening of the muscles.3. Myosin of normal, self‐innervated, and cross‐innervated muscles combined with F‐actin superprecipitated at rates which were proportional to the speed of muscle contraction.4. The pH profile curve and the ATP‐induced dinitrophenylation reaction revealed that the structure of myosin of X‐EDL was altered to that of N‐SOL or S‐SOL, and the structure of myosin of X‐SOL was modified to that of N‐EDL or S‐EDL.5. No differences were found in the yield of myosin of normal, self‐innervated, and cross‐innervated extensor digitorum longus and soleus muscles.