Antigen-provoked increase in histamine reactivity. Observations on mechanisms.
Antigen-provoked increase in histamine reactivity. Observations on mechanisms.
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抗原引起的组胺反应性增加。
DOI：
10.1164/ajrccm/144.3_pt_1.642
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Naclerio,RM
中科院分区：
文献类型：
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作者：
Walden,SM;Proud,D;Lichtenstein,LM;Kagey-Sobotka,A;Naclerio,RM
为了研究抗原激发对组胺非特异性反应性的影响，9名过敏个体接受了多次组胺或抗原鼻腔刺激。通过计算打喷嚏次数和测定回收的鼻灌洗液中白蛋白和TAME-酯酶（TAME； [3H]N-α- toyl - l-精氨酸甲酯）活性水平来评估对刺激的反应。在抗原的情况下，组胺水平也被测量。作为对组胺的反应，受试者打喷嚏，白蛋白和TAME水平升高。组胺刺激后24 h对抗原或组胺的反应无变化。然而，在抗原激发24小时后，与基线刺激相比，组胺激发的反应在症状评分和鼻灌洗液中TAME活性和白蛋白水平方面有所增加。从1到3天增加抗原暴露并没有进一步提高对组胺的反应性。暴露抗原12天后，组胺反应性恢复到基线水平。抗原刺激后和组胺刺激前灌洗液中嗜酸性粒细胞、中性粒细胞和阿利新蓝阳性细胞的数量与组胺反应的变化相关。抗原刺激引起的打喷嚏次数增加与组胺激发后24小时组胺激发后相同参数的增加相关（r = 0.88, p < 0.01）。令人惊讶的是，评估的其他参数并没有显示出类似的相关性。我们得出结论：(1)组胺激发不会引起对自身或对抗原的反应性增加；(2)抗原诱导与炎症细胞浸润相关的组胺反应性可逆增加；(3)抗原诱导的特异性（抗原）和非特异性（组胺）反应性增加之间存在微弱关系。
To investigate the effect of antigen provocation on nonspecific reactivity to histamine, nine allergic individuals underwent multiple nasal challenges with histamine or antigen. The response to challenge was assessed by counting the number of sneezes and measuring the levels of albumin and TAME-esterase (TAME; [3H]N-α-tosyl-L-arginine methyl ester) activity in recovered nasal lavages. In the case of antigen, levels of histamine were also measured. In response to histamine, the subjects sneezed and had increased levels of albumin and TAME. The responses to antigen or histamine were unchanged 24 h after histamine provocation. The responses to histamine provocations were increased with respect to symptom scores and to the levels of TAME activity and albumin in nasal lavages compared to baseline challenge, however, 24 h after antigen provocation. Increasing antigen exposure from 1 to 3 days did not further increase the responsiveness to histamine. Histamine reactivity returned to baseline 12 days after antigen exposure. The number of eosinophils, neutrophils, and alcian blue-positive cells in the lavages after antigen challenge and before the histamine challenges correlated with the changes in response to histamine. The increase in sneezing induced by antigen stimulation correlated with the increase in the same parameter after histamine provocation 24 hr after antigen challenge (r = 0.88; p < 0.01). Surprisingly, the other parameters assessed did not show similar correlations. We conclude that (1) histamine provocation does not induce increased reactivity to itself or to antigen, (2) antigen induces a reversible increase in responsiveness to histamine that is associated with an inflammatory cell infiltration, and (3) there is a weak relationship between an antigen-induced increase in specific (antigen) and nonspecific (histamine) reactivity.