Light-microscopic analysis of sectioned Sézary cells: an accurate alternative to electron microscopy.
Light-microscopic analysis of sectioned Sézary cells: an accurate alternative to electron microscopy.
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Sézary 细胞切片的光学显微镜分析：电子显微镜的准确替代方案。
DOI：
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发表时间：
1980
影响因子：
6
通讯作者：
D. Ramsey
中科院分区：
文献类型：
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作者：
C. Myrie;D. Zucker‐Franklin;D. Ramsey
蕈样肉芽肿（MF）中循环Sézary细胞的预后意义尚不清楚，MF或Sézary综合征中Sézary细胞计数波动的意义尚未评估。由于常规血涂片计数不准确，以及常规用途的电子显微镜不可用，这些研究受到阻碍。目前对35例MF或Sézary综合征患者的外周血进行的研究表明，通过切片Epon包埋淋巴细胞组分的光学显微镜进行的Sézary细胞计数与在超微结构水平进行的计数一样准确。此外，这些研究包括对20名患者的初步观察，这些患者的Sézary细胞计数在3个月至5年以上的时间间隔内重复进行。所述方法应有助于在更大范围内进行血液和淋巴结Sézary细胞计数，如果要评价循环Sézary细胞的意义，则必须进行这种计数。
The prognostic implications of circulating Sézary cells in mycosis fungoides (MF) are not known, and the significance of fluctuating Sézary cell counts in either MF or the Sézary syndrome has not been assessed. Such studies have been hampered by the inaccuracy of counts performed on routine blood smears and the unavailability of electron microscopy for routine purposes. The present studies conducted on the peripheral blood of 35 patients with either MF or the Sézary syndrome show that Sézary cell counts performed by light microscopy of sectioned Epon-embedded lymphocyte fractions are as accurate as those carried out at the ultrastructural level. In addition, the studies include preliminary observations concerning 20 patients whose Sézary cell counts were repeated over time intervals ranging from 3 months to over 5 years. The described method should facilitate the performance of blood and lymph node Sézary cell counts on a wider scale, which is a necessity if the significance of circulating Sézary cells is to be evaluated.