Parylene neurocages for electrical stimulation on silicon and glass substrates.
Parylene neurocages for electrical stimulation on silicon and glass substrates.
复制标题
用于硅和玻璃基板上电刺激的聚对二甲苯神经笼。
DOI：
10.1109/iembs.2006.260472
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Pine,Jerry
中科院分区：
文献类型：
--
作者：
Tooker,Angela;Erickson,Jon;Chow,Gary;Tai,Yu-Chong;Pine,Jerry
我们提出了一种改进的方法和设计，用于构建聚对二甲苯神经笼，用于活神经网络的体外研究。Parylene神经笼具有生物相容性并且非常坚固，使其非常适合研究单个神经元之间的突触连接，以深入了解学习和记忆。神经笼制造过程将电极并入神经笼中以允许刺激和记录动作电位。这些神经笼可以在硅或玻璃基板上制造。由此产生的神经笼具有约50%的长期细胞存活率，并且已被证明在捕获神经元方面有99%的有效性。初步结果表明，通过电极的电流脉冲可以刺激被困在神经笼中的神经元的动作电位
We present a refined method and design for building parylene neurocages for in vitro studies of live neural networks. Parylene neurocages are biocompatible and very robust, making them ideally suited for studying the synaptic connections between individual neurons to gain insight into learning and memory. The neurocage fabrication process incorporates electrodes into the neurocages to allow for stimulation and recording of action potentials. These neurocages can be fabricated on either silicon or glass substrates. The resulting neurocages have a long term cell survival rate of ~50%, and have proven to be 99% effective in trapping neurons. Preliminary results demonstrate that current pulses passing through the electrode can stimulate action potentials in the neurons trapped in neurocages