Sox2 is the Faithful Marker for Pluripotency in Pig: Evidence From Embryonic Studies
Sox2 is the Faithful Marker for Pluripotency in Pig: Evidence From Embryonic Studies
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Sox2 是猪多能性的忠实标记：来自胚胎研究的证据
DOI：
10.1002/dvdy.24248
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发表时间：
2015-04-01
影响因子：
2.5
通讯作者：
Liu, Zhonghua
中科院分区：
文献类型：
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作者：
Liu, Shichao;Bou, Gerelchimeg;Liu, Zhonghua
背景：哺乳动物第一系分离产生滋养外胚层（TE）和多能性内细胞团（ICM），为研究多能性维持和丧失机制提供了理想的模型。在小鼠中，转录因子OCT4限制ICM，并在TE/ICM规范和多能调控网络中发挥关键作用。然而，在猪中，OCT4并不局限于ICM细胞，这表明TE/ICM规范和多能调控网络的分子基础不同。为了探究猪TE/ICM的分子调控机制，我们检测了多能性因子SOX2、REX1、SALL4、ESG1、NANOG、TBX3、LIN28、KLF2和KLF5在猪囊胚中的表达模式。我们发现SOX2是一个忠实的多能性标记物，随着胚胎部分细胞、ICM细胞和新生EPI细胞的发育过程而锚定在多能细胞上，而OCT4几乎同时在所有细胞中表达。SOX2与TE标志物CDX2在D6囊胚中的时空分布一致，而OCT4与CDX2在同一时期无相关性。我们的研究结果为进一步研究猪多能调控网络提供了分子基础和线索。科学通报，2015,(4):619-627。(c) 2015 Wiley期刊公司
Background: Mammalian first lineage segregation generates trophectoderm (TE) and pluripotent inner cell mass (ICM), which provides an ideal model for studying the mechanisms of maintenance and loss of pluripotency. In mouse, the transcription factor OCT4 restricts to ICM and plays a key role in TE/ICM specification and pluripotent regulatory networks. However, in pig, OCT4 does not restrict to ICM cells, suggesting a different molecular basis in TE/ICM specification and pluripotent regulatory networks. : To explore molecular basis of porcine TE/ICM specification and pluripotent regulatory networks, we examined expression pattern of pluripotency factors, including SOX2, REX1, SALL4, ESG1, NANOG, TBX3, LIN28, KLF2, and KLF5, in porcine blastocysts. We found that SOX2 is a faithful pluripotent marker that anchored to the pluripotent cells including embryonic part cells, ICM cells and newly EPI cells along with developmental progress, whereas OCT4 expressed in almost all the cells at the same time. Consistently, analysis of spatiotemporal distribution of SOX2 and the TE marker CDX2 revealed an exclusive expression pattern in D6 blastocysts, whereas no correlation was observed between OCT4 and CDX2 at the same stage. : Our results provide a molecular basis in porcine embryonic patterning and a clue for further studying porcine pluripotent regulatory networks. Developmental Dynamics 244:619-627, 2015. (c) 2015 Wiley Periodicals, Inc.