The effect of prostaglandins on the release of arginine vasopressin from the guinea pig hypothalamo-neurohypophyseal complex in organ culture.
The effect of prostaglandins on the release of arginine vasopressin from the guinea pig hypothalamo-neurohypophyseal complex in organ culture.
复制标题
前列腺素对器官培养中豚鼠下丘脑-神经垂体复合体释放精氨酸加压素的影响。
作者：
S. Ishikawa;T. Saito;S. Yoshida
在器官培养的雄性豚鼠下丘脑-神经垂体复合体（HNC）上观察了洋地黄素（PGs）对精氨酸加压素（AVP）释放的影响。HNC外植体在培养的第二天使用。培养基中含有PGE 2引起的AVP释放从外植体在10分钟的孵育期间增加。当PGE 2浓度为2 × 10（-8）M或更高时，AVP释放显著增加。AVP释放呈剂量依赖性增加。1.4 × 10 ~（-7）和1.4 × 10 ~（-6）M浓度的PGF_（2 α）也能显著增加AVP的释放。在实验前60分钟用7.4 × 10（-5）M吲哚美辛处理的组中的外植体和在整个实验中对6.5 × 10（-8）M血管紧张素II有反应，AVP释放比对照释放增加166 +/- 42%。与未用吲哚美辛处理的组相比，该组中外植体对添加血管紧张素II的反应显著较低（P <0.05），为基础值的439 +/- 128%（P <0.05）。在310 mosmol/kg H2O的高渗氯化钠溶液中，吲哚美辛治疗组AVP的释放为基础值的120 +/- 15%，显著低于（P <0.05）未用吲哚美辛治疗组（基础值的203 +/- 30%; P <0.005）。然而，吲哚美辛并不改变AVP的基础释放。这些结果表明，在器官培养中，PGE 2和PGF 2 α可增加HNC外植体的AVP释放，尽管PGF 2 α的作用相对弱于PGE 2，并且PGs可调节AVP释放对血管紧张素II和渗透刺激的响应。
The effect of prostaglandins (PGs) on arginine vasopressin (AVP) release was investigated using the male guinea pig hypothalamo-neurohypophyseal complex (HNC) in organ culture. The explants of HNC were used on the second day in culture. The culture medium containing PGE2 caused an increase in AVP release from the explants during a 10-min incubation period. AVP release was significantly increased at a PGE2 concentration of 2 X 10(-8) M or more. AVP release was increased in a dose-dependent manner. PGF2 alpha at concentrations of 1.4 X 10(-7) and 1.4 X 10(-6) M also increased AVP release significantly. The explants in the group treated with 7.4 X 10(-5) M indomethacin for 60 min before the experiment and throughout the experiment responded to 6.5 X 10(-8) M angiotensin II with a 166 +/- 42% increase in AVP release over control release. The response of explants to the addition of angiotensin II in this group was significantly lower (P less than 0.05) than that in the group not treated with indomethacin, which was 439 +/- 128% of the basal value (P less than 0.05). The release of AVP in the group treated with indomethacin was 120 +/- 15% of the basal value in response to a hypertonic solution of sodium chloride at 310 mosmol/kg H2O and was significantly lower (P less than 0.05) than that in the group not treated with indomethacin (203 +/- 30% of the basal value; P less than 0.005). However, indomethacin did not alter basic release of AVP. These results suggest that PGE2 and PGF2 alpha increase AVP release from the explants of HNC in organ culture, although PGF 2 alpha has a relatively weaker effect than PGE2, and PGs may modulate the regulation of AVP release in response to angiotensin II and osmotic stimulation.