The Simultaneous Determination of Tricarboxylic Acid Cycle Acids and 2-Hydroxyglutarate in Serum from Patients with Nasopharyngeal Carcinoma Via GC-MS
The Simultaneous Determination of Tricarboxylic Acid Cycle Acids and 2-Hydroxyglutarate in Serum from Patients with Nasopharyngeal Carcinoma Via GC-MS
复制标题
GC-MS同时测定鼻咽癌患者血清中三羧酸循环酸和2-羟基戊二酸
DOI：
10.1007/s10337-016-3061-9
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发表时间：
2016
期刊：
影响因子：
1.7
通讯作者：
Yongguang Tao
中科院分区：
文献类型：
--
作者：
Xiaozhen He;Shao Liu;Weiwei Lai;Bin Yan;Xiaoli Liu;Yiqun Jiang;Shuang Liu;Ling Chen;Ying Shi;Yongguang Tao
The tricarboxylic acid (TCA) cycle has recently been in the spotlight in the field of oncology. Mutations in the enzymes called cytosolic isocitrate dehydrogenases (IDHs) are a common feature in several types of cancers and provide the enzyme with the new ability to catalyze α-ketoglutarate (αKG) to 2-hydroxyglutarate (2HG), which is now considered to be a major contributor to the oncogenic activity of IDHs mutations. In this study, we describe the development and full validation of a gas chromatography–mass spectrometry (GC–MS) method for the simultaneous quantitative determination of serum TCA cycle metabolites and 2HG in nasopharyngeal carcinoma serum in the same workflow. All eight organic acids observed in the serum sample showed good linearity in the corresponding linear range during the same run. The linear ranges of the method were 10–680 ng mL−1for succinic acid, 10–640 ng mL−1for fumaric acid, 10–520 ng mL−1for malic acid, 40–860 ng mL−1for oxaloacetic acid, 36–780 ng mL−1for 2HG, 38–840 ng mL−1for αKG, 40–960 ng mL−1for aconitic acid and 68–6800 ng mL−1for citric acid. The recovery and reproducibility were satisfactory, and the data demonstrate that the method is simple, sensitive, and suitable for nasopharyngeal carcinoma serum analysis.
