Induction and verification of autotetraploids in diploid banana (Musa acuminata) by in vitro techniques
Induction and verification of autotetraploids in diploid banana (Musa acuminata) by in vitro techniques
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DOI：
10.1007/bf00029262
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发表时间：
1996-01-01
期刊：
影响因子：
1.9
通讯作者：
Afza, R
中科院分区：
文献类型：
--
作者：
vanDuren, M;Morpurgo, R;Afza, R
采用流式细胞术和口孔特征技术筛选了大量体外诱导的尖沙Musa育种无性系SH-3362的自多倍体倍性水平。茎尖在添加5.0 mM秋水仙碱的液体培养基中培养48小时，或在添加2% (v/v) DMSO的液体培养基中培养30亩M oryzalin（3,5-二硝基- n4， n-二丙基硫酸酯）培养7天，在第4营养代产生非嵌合四倍体的频率分别为23.1%和29.1%。虽然混合倍性在随后的无性繁殖周期中持续存在，但通过流式细胞术鉴定的四倍体在另外两个周期中仍保持固体非嵌合性。这些同源四倍体繁殖用于田间试验。根据V4植株的气孔特征对其再生植株进行了粗略的预选，结果表明该群体中只有56.2%的植株是实心四倍体。本文报道的体细胞多倍体系统可用于香蕉育种计划。
Flow cytometry and stomata characteristics were used for screening ploidy levels in a large population of in vitro induced autopolyploids of the Musa acuminata breeding clone SH-3362. Culturing shoot tips in liquid medium supplemented both with 5.0 mM colchicine for 48 hours or 30 mu M oryzalin (3,5-dinitro-N4,N-dipropylsulphate) for seven days, both in combination with 2% (v/v) DMSO, resulted in a high (23.1% and 29.1%) frequency of non-chimeric tetraploids in the fourth vegetative generation. Although mixoploidy persisted in subsequent cycles of vegetative propagation, tetraploids as identified by flow cytometry remained solid non-chimeric during two more cycles. These autotetraploids were propagated for field testing. A rough pre-selection of regenerated V4 plants based on their stomata characteristics resulted in a population in which only 56.2% of the plants were solid tetraploids. The somatic polyploidization system reported here can be utilised for banana breeding programmes.