Co-exposure to polycyclic aromatic hydrocarbons, benzene and toluene and their dose-effects on oxidative stress damage in kindergarten-aged children in Guangzhou, China
Co-exposure to polycyclic aromatic hydrocarbons, benzene and toluene and their dose-effects on oxidative stress damage in kindergarten-aged children in Guangzhou, China
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广州市幼儿园学龄前儿童多环芳烃、苯和甲苯的共同暴露及其剂量对氧化应激损伤的影响
DOI：
10.1016/j.scitotenv.2015.04.020
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发表时间：
2015-08-15
影响因子：
9.8
通讯作者：
Fan, Ruifang
中科院分区：
文献类型：
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作者：
Li, Junnan;Lu, Shaoyou;Fan, Ruifang
多环芳烃（PAHs）、苯和甲苯（BT）是环境中普遍存在的有毒污染物。儿童对这些污染物非常敏感。为探讨多环芳烃（PAHs）和BT联合暴露对儿童DNA的潜在氧化损伤，选取广州市某幼儿园87名3-6岁儿童为研究对象。采用液相色谱串联质谱仪测定了10种尿中多环芳烃和4种BT代谢物以及尿中8-羟基-2 '-脱氧鸟苷（8-OHdG，氧化DNA损伤的生物标志物）。结果表明，广州市儿童体内多环芳烃和苯并三氮唑的含量是国外儿童的2-30倍。特别是芘和挥发性BT的差异更大。多环芳烃和BT的联合暴露可导致DNA氧化损伤。尿中多环芳烃单羟基代谢物（SOH-PAH）的总浓度、BT（Sigma BT）的总浓度和8-OHdG水平之间观察到显著的剂量效应。尿中多环芳烃和BT每增加1%，尿中8-OHdG分别增加0.33%和0.02%。我们还确定了多环芳烃和BT的尿液水平与儿童的年龄呈负相关。此外，3岁和6岁儿童SOH-PAHs和Sigma BT水平存在显著差异（p < 0.05），这可能是由于代谢能力或吸入频率不同造成的。总之，暴露于多环芳烃或BT可导致DNA氧化损伤，8-OHdG是一个很好的生物标志物，指示DNA损伤的存在。PAH暴露、BT暴露与尿中8-OHdG浓度之间存在显著的剂量-效应关系。与年龄较大的儿童相比，幼儿（3-4岁）面临更高的PAH和BT暴露负担。(C)2015爱思唯尔B. V.保留所有权利。
Polycyclic aromatic hydrocarbons (PAHs), benzene and toluene (BT) are ubiquitous toxic pollutants in the environment. Children are sensitive and susceptible to exposure to these contaminants. To investigate the potential oxidative DNA damage from the co-exposure of PAHs and BT in children, 87 children (aged 3-6) from a kindergarten in Guangzhou, China, were recruited. Ten urinary PAHs and four BT metabolites, as well as 8-hydroxy-2'-deoxyguanosine (8-OHdG, a biomarker of oxidative DNA damage) in urine, were determined using a liquid chromatography tandem mass spectrometer. The results demonstrated that the levels of PAHs and BT in children from Guangzhou were 2-30 times higher than those in children from the other countries based on a comparison with recent data from the literature. In particular, the difference is more substantial for pyrene and volatile BT. Co-exposure to PAHs and BT could lead to additive oxidative DNA damage. Significant dose-effects were observed between the sum concentration of urinary monohydroxylated metabolites of PAHs (SOH-PAHs), the sum concentration of the metabolites of BT (Sigma BT) and 8-OHdG levels. Every one percent increase in urinary PAHs and BT generated 0.33% and 0.02% increases in urinary 8-OHdG, respectively. We also determined that the urinary levels of PAHs and BTwere negatively associated with the age of the children. Moreover, significant differences in the levels of SOH-PAHs and Sigma BT were determined between 3- and 6-year-old children (p < 0.05), which may be caused by different metabolism capabilities or inhalation frequencies. In conclusion, exposure to PAHs or BT could lead to oxidative DNA damage, and 8-OHdG is a good biomarker for indicating the presence of DNA damage. There exists a significant dose-effect relationship between PAH exposure, BT exposure and the concentration of 8-OHdG in urine. Toddlers (3-4 years old) face a higher burden of PAH and BT exposure compared with older children. (C) 2015 Elsevier B.V. All rights reserved.