STAGES OF OOCYTE DEVELOPMENT IN THE ZEBRAFISH, BRACHYDANIO-RERIO
STAGES OF OOCYTE DEVELOPMENT IN THE ZEBRAFISH, BRACHYDANIO-RERIO
复制标题
DOI：
10.1002/jmor.1052180209
复制
发表时间：
1993-11-01
影响因子：
1.5
通讯作者：
QI, XP
中科院分区：
文献类型：
--
作者：
SELMAN, K;WALLACE, RA;QI, XP
基于形态学标准以及生理和生化事件，斑马鱼（Brachydanio rerio）的卵母细胞发育被分为五个阶段。在第一阶段（初级生长阶段），卵母细胞与其他卵母细胞共同位于卵巢中（IA期），然后位于一个确定的卵泡内（IB期），在此期间它们的体积大幅增大。在第二阶段（皮质小泡期），卵母细胞因出现大小不一的皮质小泡而易于区分，并且卵黄膜变得明显。在第三阶段（卵黄生成期），卵母细胞中出现卵黄蛋白，在这个主要的生长阶段，含有结晶卵黄的卵黄体逐渐积累。卵母细胞具备了在体外对类固醇17α,20β - 二羟基 - 4 - 孕烯 - 3 - 酮（DHP）作出反应并进行卵母细胞成熟的能力。在第四阶段（卵母细胞成熟阶段），卵母细胞体积略有增大，变得半透明，并且在体内进行最终减数分裂成熟时（以及在体外对DHP作出反应时）其卵黄变为非结晶状态。在第五阶段（成熟卵子），卵子（约0.75毫米）被排入卵巢腔并能够受精。这个分期系列为未来研究斑马鱼卵母细胞发育过程中的细胞过程奠定了基础，并且对于需要了解特定阶段事件的实验应该是有用的。（C）1993威利 - 利斯公司
Oocyte development has been divided into five stages in the zebrafish Brachydanio rerio, based on morphological criteria and on physiological and biochemical events. In stage I (primary growth stage), oocytes reside in nests with other oocytes (Stage IA) and then within a definitive follicle (Stage IB), where they greatly increase in size. In stage II (cortical alveolus stage), oocytes are distinguished by the appearance of variably sized cortical alveoli and the vitelline envelope becomes prominent. In stage III (vitellogenesis), yolk proteins appear in oocytes and yolk bodies with crystalline yolk accrue during this major growth stage. Oocytes develop the capacity to respond in vitro to the steroid 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP) by undergoing oocyte maturation. In stage IV (oocyte maturation), oocytes increase slightly in size, become translucent, and their yolk becomes non-crystalline as they undergo final meiotic maturation in vivo (and in response to DHP in vitro). In stage V (mature egg), eggs (approx. 0.75 mm) are ovulated into the ovarian lumen and are capable of fertilization. This staging series lays the foundation for future studies on the cellular processes occurring during oocyte development in zebrafish and should be useful for experimentation that requires an understanding of stage-specific events. (C) 1993 Wiley-Liss, Inc.