Keishi-bukuryo-gan preserves the endothelium dependent relaxation of thoracic aorta in cholesterol-fed rabbit by limiting superoxide generation
Keishi-bukuryo-gan preserves the endothelium dependent relaxation of thoracic aorta in cholesterol-fed rabbit by limiting superoxide generation
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DOI：
10.1002/ptr.945
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发表时间：
2002-09-01
影响因子：
7.2
通讯作者：
Terasawa, K
中科院分区：
文献类型：
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作者：
Sekiya, N;Goto, H;Terasawa, K
本研究旨在探讨庆大霉素（Keishi-bukuryo-gan，KBG）对动脉粥样硬化的早期抑制作用。在研究开始和结束时，评价中性粒细胞中促凝剂爆发期间的血浆脂质浓度和羟基自由基生成。研究了KBG对高胆固醇血症引起的内皮功能紊乱的保护作用。将12只雄性日本白色家兔（体重2 kg）分为两组。A组（n = 6）喂饲含1%胆固醇的标准兔饲料4周。B组（n = 6）喂饲含1%胆固醇和1%KBG的标准兔饲料4周。在血脂浓度中，只有A组的脂质过氧化物浓度显著高于B组。研究结束时，两组中性粒细胞产生的羟基自由基的自旋捕获加合物DMPO-OH均增加，B组增加明显。与A组相比，B组乙酰胆碱依赖性血管舒张作用显著增强。因此，KBG通过其抗氧化作用和抑制体内中性粒细胞释放自由基来保护血管内皮功能。版权所有（C）2002约翰威利父子有限公司
Formerly, we have reported that keishi-bukuryo-gan prevents the progression of atherosclerosis in cholesterol-fed rabbits and inhibits the free radical-induced RBC haemolysis in rats.The present study was performed to investigate how keishi-bukuryo-gan (KBG) inhibits the early stage of atherosclerosis. Plasma lipid concentration and hydroxyl radical generation during respirators burst in neutrophils were evaluated at the start and end of the study. The protective effect of KBG against endothelium disorder due to hypercholesterolaemia was examined. Twelve male Japanese white rabbits (2 kg body weight) were divided into two groups. Group A (n = 6) was fed standard rabbit chow containing 1% cholesterol for 4 weeks. Group B (n = 6) was fed standard rabbit chow containing 1% cholesterol and 1% KBG for 4 weeks. In the plasma lipid concentration, only the lipid peroxide concentration of group A was significantly higher than that of group B. At the end of the study, DMPO-OH, the spin-trapped adduct of hydrox I radicals generated by neutrophils, was increased in both groups, and this increase was marked in group B. Endothelium-dependent vasodilatation by acetylcholine increased significantly in group B compared with group A. Thus, KBG protects the vascular endothelium function by its antioxidative effect and by inhibiting the release of free radicals from neutrophils in vivo. Copyright (C) 2002 John Wiley Sons, Ltd.