Effects of demecolcine and sucrose on the incidence of cytoplasmic protrusions containing chromosomes in pig oocytes matured in vitro
Effects of demecolcine and sucrose on the incidence of cytoplasmic protrusions containing chromosomes in pig oocytes matured in vitro
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DOI：
10.1262/jrd.19142
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发表时间：
2008-04-01
影响因子：
1.8
通讯作者：
Yoshida, Mitsutoshi
中科院分区：
文献类型：
--
作者：
Miyoshi, Kazuchika;Mori, Hironori;Yoshida, Mitsutoshi
本研究旨在探讨去甲花青素和蔗糖是否单独或联合影响猪卵母细胞中含有染色体的细胞质突起的形成。在蔗糖浓度为20 mM的条件下，0.2~1.0mU g/ml地霉素组培养60min的卵母细胞胞浆突起率显著高于0.01~0.05mU g/mldM组。当卵母细胞在0.2mU g/ml去甲氧胆碱和20 mM蔗糖中培养15min时，35.1%的卵母细胞有胞质突起，显著低于培养30~90min的卵母细胞。在0.2 mU g/ml地麦角碱存在下，160 mM蔗糖培养30min的卵母细胞胞质突起数显著少于0-80 mM蔗糖培养30min。无论是否加入20 mM蔗糖，加入0.2 mU g/ml地麦角新碱培养30min后，卵母细胞均有明显的胞浆突起。在88.9-100%的卵母细胞中，胞质突起含有染色体，在不同浓度的去甲甘露糖和蔗糖以及不同处理时间之间没有显著差异。本研究结果表明，猪卵母细胞中含有染色体的细胞质突起与去甲胆碱有关，但蔗糖不影响其形成。
The present study was carried out to examine whether demecolcine and sucrose affect the formation of a cytoplasmic protrusion containing chromosomes in pig oocytes independently or in combination. In the presence of 20 mM sucrose, the rates of oocytes with a cytoplasmic protrusion after culture for 60 min with 0.2-1.0 mu g/ml demecolcine were significantly higher than those with 0.01-0.05 mu g/ml demecolcine. When oocytes were cultured for 15 min in the presence of 0.2 mu g/ml demecolcine and 20 mM sucrose, 35.1% of them extruded a cytoplasmic protrusion; this rate was significantly lower than those of oocytes cultured for 30-90 min. In the presence of 0.2 mu g/ml demecolcine, significantly fewer oocytes extruded a cytoplasmic protrusion after culture for 30 min with 160 mM sucrose than with 0-80 mM sucrose. Significantly more oocytes extruded a cytoplasmic protrusion after culture for 30 min with 0.2 mu g/ml demecolcine than without it, regardless of the presence or absence of 20 mM sucrose. In 88.9-100% of the oocytes, the cytoplasmic protrusions contained chromosomes with no significant differences among the different concentrations of demecolcine and sucrose and among the different treatment times. The results of the present study show that the cytoplasmic protrusion containing chromosomes in the pig oocyte is attributable to demecolcine, but sucrose does not affect its formation.