Insights into the mechanism of the interference of sulfadiazine on soil microbial community and function.
Insights into the mechanism of the interference of sulfadiazine on soil microbial community and function.
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DOI：
10.1016/j.jhazmat.2021.126388
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发表时间：
2021-06
影响因子：
13.6
通讯作者：
Linlin Qiu;T. Daniell;S. Banwart;M. Nafees;Jingjing Wu;Wenchao Du;Ying Yin;Hongyan Guo
中科院分区：
文献类型：
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作者：
Linlin Qiu;T. Daniell;S. Banwart;M. Nafees;Jingjing Wu;Wenchao Du;Ying Yin;Hongyan Guo
磺胺类药物在土壤环境中的累积具有改变土壤微生物群落结构和功能的潜力。代谢组学能够提供对碳代谢池和与外部压力相关的分子机制的见解。本文研究了磺胺嘧啶（SDZ）暴露对土壤细菌群落和土壤代谢产物的影响，并提出了SDZ在土壤中积累影响土壤有机质（SOM）循环的可能机制。测序分析表明，与碳循环相关的细菌物种的相对丰度在高浓度的SDZ暴露下显着下降。非靶向代谢组学分析表明，SDZ在土壤中的存在使78种代谢产物发生了显著变化。功能预测和途径分析的结合都表明，高浓度的SDZ暴露可引起的前、后神经元功能紊乱。此外，在高浓度的SDZ暴露下，碳水化合物的相对含量显着下降，可能会削弱物理分离，并为微生物提供更多的机会来降解有机质。上述结果证明SDZ在土壤中的积累有可能扰乱SOM循环。这些发现扩展了我们对抗生素在土壤环境中的环境风险的理解，而不仅仅是抗生素耐药性的传播。
The accumulation of sulfonamides in the soil environment possessed the potential to change soil microbial community and function. Metabolomics is capable of providing insights into the carbon metabolic pool and molecular mechanisms associated with external stressors. Here we evaluated alternations in soil bacterial community and soil metabolites profiles under sulfadiazine (SDZ) exposure and proposed a potential mechanism that SDZ accumulation in soil affected soil organic matter (SOM) cycling. Sequencing analysis showed that the relative abundance of bacterial species associated with carbon cycling significantly decreased under high concentrations of SDZ exposure. Untargeted metabolomics analysis showed that 78 metabolites were significantly changed with the presence of SDZ in soil. The combination of functional predictions and pathway analysis both demonstrated that high concentrations of SDZ exposure could cause disturbance in anabolism and catabolism. Moreover, the noticeable decline in the relative content of carbohydrates under high concentrations of SDZ exposure might weaken physical separation and provide more chances for microbes to degrade SOM. The above results provided evidence that SDZ accumulation in soil held the potential to disturb SOM cycling. These findings spread our understanding about the environmental risk of antibiotic in the soil environment beyond the dissemination of antibiotic resistance.