Urea and urine are a viable and cost-effective nitrogen source for Yarrowia lipolytica biomass and lipid accumulation
Urea and urine are a viable and cost-effective nitrogen source for Yarrowia lipolytica biomass and lipid accumulation
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DOI：
10.1007/s00253-018-8769-z
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发表时间：
2018-03-01
影响因子：
5
通讯作者：
Blenner, Mark A.
中科院分区：
文献类型：
--
作者：
Brabender, Matthew;Hussain, Murtaza Shabbir;Blenner, Mark A.
解脂Yarrowia是一种工业酵母，由于其高生长能力、遗传易处理性和含油性，已被用于可持续生产脂肪酸衍生化合物和脂类化合物。本研究探讨了利用尿素或尿液替代硫酸铵作为氮源培养溶脂耶尔森菌的可能性。当以葡萄糖为碳源时，用化学计量当量的尿素代替硫酸铵显著促进了细胞的生长。此外，用人工尿液和真人尿液培养时，解脂耶尔森菌的生长同样得到改善。当细胞生长在尿素或尿液中时，可以获得等量或更好的脂肪生成。成功地将尿素和尿液用作解脂耶尔森菌生长的氮源，突出了使用更便宜的培养基组分以及为生物技术过程开发和回收未经处理的人类排泄物的潜力。
Yarrowia lipolytica is an industrial yeast that has been used in the sustainable production of fatty acid-derived and lipid compounds due to its high growth capacity, genetic tractability, and oleaginous properties. This investigation examines the possibility of utilizing urea or urine as an alternative to ammonium sulfate as a nitrogen source to culture Y. lipolytica. The use of a stoichiometrically equivalent concentration of urea in lieu of ammonium sulfate significantly increased cell growth when glucose was used as the carbon source. Furthermore, Y. lipolytica growth was equally improved when grown with synthetic urine and real human urine. Equivalent or better lipid production was achieved when cells are grown on urea or urine. The successful use of urea and urine as nitrogen sources for Y. lipolytica growth highlights the potential of using cheaper media components as well as exploiting and recycling non-treated human waste streams for biotechnology processes.