Functional interaction between RNA polymerase α subunit C-terminal domain and σ70 in UP-element- and activator-dependent transcription
Functional interaction between RNA polymerase α subunit C-terminal domain and σ70 in UP-element- and activator-dependent transcription
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DOI：
10.1016/s1097-2765(03)00201-6
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发表时间：
2003-06-01
期刊：
影响因子：
16
通讯作者：
Ebright, RH
中科院分区：
文献类型：
--
作者：
Chen, H;Tang, H;Ebright, RH
我们表明，大肠杆菌 RNA 聚合酶 (RNAP) 亚基 C 端结构域 (alphaCTD) 在 UP 元件和激活剂依赖性启动子子集上与 sigma(70) 功能性地相互作用，我们定义了相互作用所需的 aCTD 和 sigma(70) 的决定因素，并提出了相互作用的结构模型。 αCTD-sigma(70)相互作用跨越上游启动子和核心启动子，从而将上游启动子中的UP元件和激活子的识别与核心启动子中的-35元件的识别联系起来。我们提出，αCTD-sigma(70) 相互作用允许 UP 元件和激活剂不仅通过与 aCTD 直接相互作用“招募”RNAP，而且还通过与 sigma(70) 间接、αCTD 桥接相互作用“重塑”RNAP-核心-启动子相互作用。
We show that the Escherichia coli RNA polymerase (RNAP) subunit C-terminal domain (alphaCTD) functionally interacts with sigma(70) at a subset of UP-element- and activator-dependent promoters, we define the determinants of aCTD and sigma(70) required for the interaction, and we present a structural model for the interaction. The alphaCTD-sigma(70) interaction spans the upstream promoter and core promoter, thereby linking recognition of UP-elements and activators in the upstream promoter with recognition of the -35 element in the core promoter. We propose that the alphaCTD-sigma(70) interaction permits UP-elements and activators not only to "recruit" RNAP through direct interaction with aCTD, but also to "remodel" RNAP-core-promoter interaction through indirect, alphaCTD-bridged interactions with sigma(70).