Universal surface-to-volume scaling and aspect ratio homeostasis in rod-shaped bacteria
Universal surface-to-volume scaling and aspect ratio homeostasis in rod-shaped bacteria
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DOI：
10.1101/583989
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发表时间：
2019-03
期刊：
影响因子：
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通讯作者：
Nikola Ojkic;D. Serbanescu;Shiladitya Banerjee
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文献类型：
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作者：
Nikola Ojkic;D. Serbanescu;Shiladitya Banerjee
杆状细菌细胞可以轻松调整其长度和宽度以响应环境变化。虽然最近的许多研究都集中在细菌细胞大小控制的机制上，但人们仍然很大程度上不清楚细胞长度和宽度之间的耦合如何导致对杆状细菌形状的稳健控制。在这项研究中，我们发现了大肠杆菌和其他杆状细菌中普遍存在的表面与体积的比例关系，这是由于细胞纵横比的保持而产生的。为了解释长宽比控制的机制起源，我们提出了细菌细胞伸长与必需分裂蛋白 FtsZ 积累之间耦合的定量模型。该模型揭示了细菌长宽比与细胞大小和生长条件无关的机制，并预测细胞形态变化对营养扰动、抗生素、MreB 或 FtsZ 消耗的响应，与实验数据定量一致。
Rod-shaped bacterial cells can readily adapt their lengths and widths in response to environmental changes. While many recent studies have focused on the mechanisms underlying bacterial cell size control, it remains largely unknown how the coupling between cell length and width results in robust control of rod-like bacterial shapes. In this study we uncover a universal surface-to-volume scaling relation in Escherichia coli and other rod-shaped bacteria, resulting from the preservation of cell aspect ratio. To explain the mechanistic origin of aspect-ratio control, we propose a quantitative model for the coupling between bacterial cell elongation and the accumulation of an essential division protein, FtsZ. This model reveals a mechanism for why bacterial aspect ratio is independent of cell size and growth conditions, and predicts cell morphological changes in response to nutrient perturbations, antibiotics, MreB or FtsZ depletion, in quantitative agreement with experimental data.